AB110643

Anti-CD34 antibody [EPR2999]

Name: Anti-CD34 antibody [EPR2999] (ab110643) | Abcam
Brand: Abcam Limited
SKU: AB110643
Price: 405 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(21 Publications)

Rabbit Recombinant Monoclonal CD34 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 21 publications.

View Alternative Names

CD34, Hematopoietic progenitor cell antigen CD34

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)

ab110643 at 1/100 staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)

ab110643 at 1/100 staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-CD34 antibody [EPR2999] (AB110643)

All lanes:

Western blot - Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution

Lane 1:

Human bone marrow tissue lysate at 10 µg

Lane 2:

TF-1 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-CD34 antibody [EPR2999] (AB110643)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Daudi, HL-60.
Low expression : HUVEC (PMID : 28994769, PMID : 19364973, and PMID : 39281105).

All lanes:

Western blot - Anti-CD34 antibody [EPR2999] (ab110643) at 1/10000 dilution

Lane 1:

KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

Lane 2:

TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

Lane 3:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 4:

HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

Lane 5:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80-120 kDa

false

Exposure time: 5s

Carrier free

Anti-CD34 antibody [EPR2999] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2999

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

See full target information CD34

style

left-column

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Hepatology forum 6:99-104 PubMed40686594

2025

Predicting hepatocellular carcinoma development in advanced fibrosis or cirrhosis due to chronic hepatitis B: The role of glypican-3, heat shock protein 70, CD34, and glutamine synthetase.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuf Ozturk,Tugrul Purnak,Halis Simsek,Cenk Sokmensuer

PloS one 20:e0325936 PubMed40601668

2025

PLZF promotes compensatory lung growth by increasing HPMEC proliferation and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Peng,Liang Ma,Zhonghui Wang,Yaxi Du,Qunfen Tao,Qiongchuan Wang,Li Zhao

Discover oncology 16:180 PubMed39948247

2025

Differences in tumor angiogenesis and related factors between lung adenocarcinomas manifesting as pure ground glass opacity and solid nodules.

Applications

Unspecified application

Species

Unspecified reactive species

Rirong Qu,Yang Zhang,Shenghui Qin,Jing Xiong,Xiangning Fu,Lequn Li,Dehao Tu,Yixin Cai

Scientific reports 13:19611 PubMed37949939

2023

Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Chuanbing Shi,Yiqiong Yang,Xiuxiu Hu,Haifeng Ni,Li Li,Zhengyuan Cheng,Jing Huang,Pingsheng Chen

Discover. Oncology 14:118 PubMed37392305

2023

Ritanserin suppresses acute myeloid leukemia by inhibiting DGKα to downregulate phospholipase D and the Jak-Stat/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinshui Tan,Mengya Zhong,Yanyan Hu,Guangchao Pan,Jingwei Yao,Yuanfang Tang,Hongpeng Duan,Yuelong Jiang,Weihang Shan,Jiaqi Lin,Yating Liu,Jiewen Huang,Huijian Zheng,Yong Zhou,Guo Fu,Zhifeng Li,Bing Xu,Jie Zha

Biomedical reports 19:50 PubMed37383678

2023

Ring finger protein 215 is a potential prognostic biomarker involved in immune infiltration and angiogenesis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Bo Wu,Xiao-Jing Li,Hui Liu,Xiu-Ping Liu

Journal of oncology 2022:8915503 PubMed36072972

2022

The Biomarker Like the Correlation between Vasculogenic Mimicry, Vascular Endothelial Cadherin, Sex-DeterminingRegion on Y-Box Transcription Factor 17, and Cyclin D1 in Oesophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yanzi Qin,Wenjun Zhao,Zhaogeng Cai,Qi Wang,Jin Gao,Hongfei Ci,Zhenzhong Feng,Li Ma

Stem cell research & therapy 13:226 PubMed35659361

2022

Human umbilical cord-derived mesenchymal stromal cells ameliorate aging-associated skeletal muscle atrophy and dysfunction by modulating apoptosis and mitochondrial damage in SAMP10 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Limei Piao,Zhe Huang,Aiko Inoue,Masafumi Kuzuya,Xian Wu Cheng

Journal of biological engineering 15:26 PubMed34819102

2021

Surface modification of decellularized bovine carotid arteries with human vascular cells significantly reduces their thrombogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Eriselda Keshi,Peter Tang,Marie Weinhart,Hannah Everwien,Simon Moosburner,Nicolai Seiffert,Michael Lommel,Ulrich Kertzscher,Brigitta Globke,Anja Reutzel-Selke,Benjamin Strücker,Johann Pratschke,Igor Maximillian Sauer,Nils Haep,Karl Herbert Hillebrandt

International journal of experimental pathology 102:268-278 PubMed34791715

2021

Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kullasit Chutipongpisit,V Praveen Parachuru,Lara T Friedlander,Haizal M Hussaini,Alison M Rich

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD34 antibody [EPR2999]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)

Western blot - Anti-CD34 antibody [EPR2999] (AB110643)

Western blot - Anti-CD34 antibody [EPR2999] (AB110643)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (21)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise