Rabbit Recombinant Monoclonal CD34 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 16 publications.
Images
![Western blot - Anti-CD34 antibody [EPR2999] (AB110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--western-blot-img35278.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD34 antibody [EPR2999] (AB110643)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132895.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130766.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (AB110643)")
![Western blot - Anti-CD34 antibody [EPR2999] (AB110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--western-blot-img445504.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD34 antibody [EPR2999] (AB110643)")
Publications
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- Scientific reports 13:196112023Identifying key genes related to the peritubular capillary rarefaction in renal interstitial fibrosis by bioinformatics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhang,Chuanbing Shi,Yiqiong Yang,Xiuxiu Hu,Haifeng Ni,Li Li,Zhengyuan Cheng,Jing Huang,Pingsheng ChenPubMed 37949939
- Discover. Oncology 14:1182023Ritanserin suppresses acute myeloid leukemia by inhibiting DGKα to downregulate phospholipase D and the Jak-Stat/MAPK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinshui Tan,Mengya Zhong,Yanyan Hu,Guangchao Pan,Jingwei Yao,Yuanfang Tang,Hongpeng Duan,Yuelong Jiang,Weihang Shan,Jiaqi Lin,Yating Liu,Jiewen Huang,Huijian Zheng,Yong Zhou,Guo Fu,Zhifeng Li,Bing Xu,Jie ZhaPubMed 37392305
- Biomedical reports 19:502023Ring finger protein 215 is a potential prognostic biomarker involved in immune infiltration and angiogenesis in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Bo Wu,Xiao-Jing Li,Hui Liu,Xiu-Ping LiuPubMed 37383678
- Journal of oncology 2022:89155032022The Biomarker Like the Correlation between Vasculogenic Mimicry, Vascular Endothelial Cadherin, Sex-DeterminingRegion on Y-Box Transcription Factor 17, and Cyclin D1 in Oesophageal Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanzi Qin,Wenjun Zhao,Zhaogeng Cai,Qi Wang,Jin Gao,Hongfei Ci,Zhenzhong Feng,Li MaPubMed 36072972
- Stem cell research & therapy 13:2262022Human umbilical cord-derived mesenchymal stromal cells ameliorate aging-associated skeletal muscle atrophy and dysfunction by modulating apoptosis and mitochondrial damage in SAMP10 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLimei Piao,Zhe Huang,Aiko Inoue,Masafumi Kuzuya,Xian Wu ChengPubMed 35659361
- Journal of biological engineering 15:262021Surface modification of decellularized bovine carotid arteries with human vascular cells significantly reduces their thrombogenicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEriselda Keshi,Peter Tang,Marie Weinhart,Hannah Everwien,Simon Moosburner,Nicolai Seiffert,Michael Lommel,Ulrich Kertzscher,Brigitta Globke,Anja Reutzel-Selke,Benjamin Strücker,Johann Pratschke,Igor Maximillian Sauer,Nils Haep,Karl Herbert HillebrandtPubMed 34819102
- International journal of experimental pathology 102:268-2782021Immunohistochemical and immunofluorescence expression profile of lymphatic endothelial cell markers in oral cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKullasit Chutipongpisit,V Praveen Parachuru,Lara T Friedlander,Haizal M Hussaini,Alison M RichPubMed 34791715
- International journal of molecular sciences 22:2021Preclinical Evaluation of Sodium Selenite in Mice: Toxicological and Tumor Regression Studies after Striatum Implantation of Human Glioblastoma Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLouis Larrouquère,Sylvie Berthier,Benoit Chovelon,Catherine Garrel,Véronique Vacchina,Hugues Paucot,Jean Boutonnat,Patrice Faure,Florence Hazane-PuchPubMed 34638987
- In vivo (Athens, Greece) 35:1437-14412021Traditional Chinese Medicine Brucea Javanica Oil Enhances the Efficacy of Anlotinib in a Mouse Model of Liver-Metastasis of Small-cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Peng,Wenhong Dong,Qiangqiang Chu,Jia Meng,Haitao Yang,Yingying DU,Y U Sun,Robert M HoffmanPubMed 33910820
- BMC cancer 20:9642020Therapeutic potential of a novel prodrug of green tea extract in induction of apoptosis via ERK/JNK and Akt signaling pathway in human endometrial cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGene Chi Wai Man,Jianzhang Wang,Yi Song,Jack Ho Wong,Yu Zhao,Tat San Lau,Kam Tong Leung,Tak Hang Chan,Huating Wang,Joseph Kwong,Tzi Bun Ng,Chi Chiu WangPubMed 33023525
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50000 - 1/200000 | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Alternative names
CD34, Hematopoietic progenitor cell antigen CD34
Recommended products
Rabbit Recombinant Monoclonal CD34 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 16 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR2999
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD34 antibody [EPR2999] (ab110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--western-blot-img35278.jpg "Western blot - Anti-CD34 antibody [EPR2999] (ab110643)")
Western blot - Anti-CD34 antibody [EPR2999] (ab110643)
All lanes: Western blot - Anti-CD34 antibody [EPR2999] (ab110643) at 1/50000 dilution
Lane 1: Human bone marrow tissue lysate at 10 µg
Lane 2: TF-1 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 120 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132895.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)
ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130766.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] (ab110643)
ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-CD34 antibody [EPR2999] (ab110643), expandable thumbnail](https://content.abcam.com/products/images/cd34-antibody-epr2999-ab110643--western-blot-img445504.jpg "Western blot - Anti-CD34 antibody [EPR2999] (ab110643)")
Western blot - Anti-CD34 antibody [EPR2999] (ab110643)
CD34 Western blot staining using rabbit Anti-CD34 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Daudi, HL-60.
Low expression: HUVEC (PMID: 28994769, PMID: 19364973, and PMID: 39281105).All lanes: Western blot - Anti-CD34 antibody [EPR2999] (ab110643) at 1/10000 dilution
Lane 1: KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg
Lane 2: TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg
Lane 3: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 4: HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg
Lane 5: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 80-120 kDa
Exposure time: 5s
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