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Rabbit Recombinant Monoclonal CD34 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

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Images

Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (AB247928), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (AB247928), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (AB247928), expandable thumbnail
  • Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (AB247928), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Alternative names

CD34, Hematopoietic progenitor cell antigen CD34

Recommended products

Rabbit Recombinant Monoclonal CD34 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR2999
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247928 is the carrier-free version of Anti-CD34 antibody [EPR2999] ab110643.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928), expandable thumbnail

    Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using Anti-CD34 antibody [EPR2999] ab110643, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CD34 antibody [EPR2999] (Anti-CD34 antibody [EPR2999] ab110643) at 1/50000 dilution

    Lane 1: Human bone marrow tissue lysate at 10 µg

    Lane 2: TF-1 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 40 kDa

    Observed band size: 120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using Anti-CD34 antibody [EPR2999] ab110643, the same antibody clone in a different buffer formulation.Anti-CD34 antibody [EPR2999] ab110643, at 1/100, staining CD34 in paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    This data was developed using Anti-CD34 antibody [EPR2999] ab110643, the same antibody clone in a different buffer formulation.Anti-CD34 antibody [EPR2999] ab110643, at 1/100, staining CD34 in paraffin-embedded Human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928), expandable thumbnail

    Western blot - Anti-CD34 antibody [EPR2999] - BSA and Azide free (ab247928)

    CD34 Western blot staining using rabbit Anti-CD34 antibody

    This data was developed using Anti-CD34 antibody [EPR2999] ab110643, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Daudi, HL-60.
    Low expression: HUVEC (PMID: 28994769, PMID: 19364973, and PMID: 39281105).

    All lanes: Western blot - Anti-CD34 antibody [EPR2999] (Anti-CD34 antibody [EPR2999] ab110643) at 1/10000 dilution

    Lane 1: KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

    Lane 2: TF-1 (human erythroleukemia erythroblast) whole cell lysate at 20 µg

    Lane 3: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 4: HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 5: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 80-120 kDa

    Exposure time: 5s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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