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Mouse Monoclonal CD34 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD34.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (AB213058), expandable thumbnail
  • Flow Cytometry - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (AB213058), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (AB213058), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human CD34. Database link P28906

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
0.5-1 µg for 106 Cells
Notes

(in 0.1 ml).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
2-4 µg/mL
Notes

(Primary incubation for 30 min at RT).

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Alternative names

Recommended products

Mouse Monoclonal CD34 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD34.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human CD34. Database link P28906
Carrier free
Yes
Clone number
HPCA1/1171
Purification technique
Affinity purification Protein G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from bioreactor concentrate.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human angiosarcoma tissue labeling CD34 with ab213058 at 4 μg/ml.

  • Flow Cytometry - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058), expandable thumbnail

    Flow Cytometry - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)

    Flow cytometric analysis of KG-1 cells labeling CD34 with ab213058 at 1 μg/106 cells (red), compared to an isotype control (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD34 with ab213058 at 4 μg/ml.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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