AB245689

Anti-CD34 antibody [My10]

Name: Anti-CD34 antibody [My10] (ab245689) | Abcam
Brand: Abcam Limited
SKU: AB245689
Price: 350 USD
Availability: InStock

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(4 Publications)

Rabbit Monoclonal CD34 antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD34 protein.

View Alternative Names

CD34, Hematopoietic progenitor cell antigen CD34

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [My10] (AB245689)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [My10] (AB245689)

Paraffin embedded human placenta tissue stained for CD34 with ab245689 at 4 μg/ml for 30 mins in immunohistochemical analysis. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3′-diaminobenzidine), and counterstaining with hemotoxylin.

Flow Cytometry - Anti-CD34 antibody [My10] (AB245689)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD34 antibody [My10] (AB245689)

Human leukocytes were stained with an isotype control (Panel A) or ab245689 (Panel B) at a concentration of 1 μg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).

Supplier Data

Western blot - Anti-CD34 antibody [My10] (AB245689)

10% SDS PAGE gel.

Detected by chemiluminescence.

All lanes:

Western blot - Anti-CD34 antibody [My10] (ab245689) at 0.1 µg/mL

All lanes:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate in RIPA buffer at 35 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

My10

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Cell preparation containing CD34 protein. The exact immunogen used to generate this antibody is proprietary information.

P28906

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "Microwave. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

See full target information CD34

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC oral health 24:407 PubMed38556862

2024

KAT2A-mediated succinylation modification of notch1 promotes the proliferation and differentiation of dental pulp stem cells by activating notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Longwei Ye,Zeqin Yu,Lin He,Jie Yuan,Xiaodan Zhang,Lei Li,Xin Huang,Yanyan Ma,Lei Zhang

Neoplasma 69:1386-1395 PubMed36591801

2023

GDF11 knockdown downregulates SMURF1 to inhibit breast cancer progression by activation of p53 and inactivation of ERα signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Li Tang,Qi Wang,Jian-Guo Lu,Xiao-Jun Yang,Jiao-Jiao Shi,Sheng-Peng Wang,Chang-Qing Cao,Hua-Dong Zhao

Frontiers in pharmacology 13:841267 PubMed35586052

2022

Targeting HMGB1-NFκb Axis and miR-21 by Glycyrrhizin: Role in Amelioration of Corneal Injury in a Mouse Model of Alkali Burn.

Applications

Unspecified application

Species

Unspecified reactive species

Peihong Wang,Peng Hao,Xi Chen,Linghan Li,Yongying Zhou,Xiaohan Zhang,Lin Zhu,Ming Ying,Ruifang Han,Liming Wang,Xuan Li

International journal of molecular medicine 47:643-658 PubMed33416146

2021

Regulatory role of DEPTOR‑mediated cellular autophagy and mitochondrial reactive oxygen species in angiogenesis in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Jizhen Wang,Junmin Chen,Dongbiao Qiu,Zhiyong Zeng

View all publications

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Product promise

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