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AB256310

Anti-CD35 antibody [7G9] - BSA and Azide free

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Mouse Recombinant Monoclonal CD35 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

View Alternative Names

CD35, C3BR, CR1, Complement receptor type 1, C3b/C4b receptor

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD35 antibody [7G9] - BSA and Azide free (AB256310)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD35 antibody [7G9] - BSA and Azide free (AB256310)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC cells labelling CD35 / CR1 with ab254151 at 1/100 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in human PBMC. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab254151 at a 1/100 dilution followed by ab150080 at a 1/500 diliution.

Negative control 2 : ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 diliution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254151).

Flow Cytometry - Anti-CD35 antibody [7G9] - BSA and Azide free (AB256310)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD35 antibody [7G9] - BSA and Azide free (AB256310)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD35 / CR1 with ab254151 at 1/1000 dilution (0.1μg) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254151 (Right), then stained with anti-CD19 conjugated to Alexa Fluor® 647.

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254151).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7G9

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab256310 is the carrier-free version of ab254151.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed : 2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed : 2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed : 2972794, PubMed : 8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed : 25742728).. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
See full target information CR1
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Product promise

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For full details, please see our Terms & Conditions

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