Rabbit Recombinant Monoclonal CD35 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] - BSA and Azide free (AB248156), expandable thumbnail](https://content.abcam.com/products/images/cd35-antibody-epr6602-bsa-and-azide-free-ab248156--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136955.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] - BSA and Azide free (AB248156)")
![Western blot - Anti-CD35 antibody [EPR6602] - BSA and Azide free (AB248156), expandable thumbnail](https://content.abcam.com/products/images/cd35-antibody-epr6602-bsa-and-azide-free-ab248156--western-blot-img99663.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD35 antibody [EPR6602] - BSA and Azide free (AB248156)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728). (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Alternative names
CD35, C3BR, CR1, Complement receptor type 1, C3b/C4b receptor
Recommended products
Rabbit Recombinant Monoclonal CD35 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6602
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248156 is the carrier-free version of Anti-CD35 antibody [EPR6602] ab126737.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156)
This data was developed using Anti-CD35 antibody [EPR6602] ab126737, the same antibody clone in a different buffer formulation.Anti-CD35 antibody [EPR6602] ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156), expandable thumbnail](https://content.abcam.com/products/images/cd35-antibody-epr6602-bsa-and-azide-free-ab248156--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136974.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156)
This data was developed using Anti-CD35 antibody [EPR6602] ab126737, the same antibody clone in a different buffer formulation.Anti-CD35 antibody [EPR6602] ab126737 at 1/250 dilution, staining CD35 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156), expandable thumbnail](https://content.abcam.com/products/images/cd35-antibody-epr6602-bsa-and-azide-free-ab248156--western-blot-img99663.jpg "Western blot - Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156)")
Western blot - Anti-CD35 antibody [EPR6602] - BSA and Azide free (ab248156)
This data was developed using Anti-CD35 antibody [EPR6602] ab126737, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CD35 antibody [EPR6602] (Anti-CD35 antibody [EPR6602] ab126737) at 1/1000 dilution
Lane 1: TF1 lysate at 10 µg
Lane 2: Human tonsil lysate at 10 µg
SecondaryAll lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 223 kDa
Downloads
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