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AB227666

Anti-CD35 antibody [SP191]

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(1 Publication)

Rabbit Recombinant Monoclonal CD35 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD35, C3BR, CR1, Complement receptor type 1, C3b/C4b receptor

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD35 with ab227666 at 1100 dilution (2.69 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227666 for 30 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)

Formalin-fixed, paraffin-embedded human kidney tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 antibody [SP191] (AB227666)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD35 using ab227666 at 1/100 dilution in immunohistochemical analysis.

  • Carrier free

    Anti-CD35 antibody [SP191] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP191

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling atroom temperature for 20 minutes.</p><p>Incubate with primary antibody for 30 minutes at room temperature.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed : 2963069). Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed : 2963069). Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed : 2972794, PubMed : 8175757). Also plays a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed : 25742728).. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
See full target information CR1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7141 PubMed39164224

2024

CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guillem Pascual-Pasto,Brendan McIntyre,Margaret G Hines,Anna M Giudice,Laura Garcia-Gerique,Jennifer Hoffmann,Pamela Mishra,Stephanie Matlaga,Simona Lombardi,Rawan Shraim,Patrick M Schürch,Mark Yarmarkovich,Ted J Hofmann,Fatemeh Alikarami,Daniel Martinez,Matthew Tsang,Luis Gil-de-Gómez,Timothy T Spear,Kathrin M Bernt,Adam J Wolpaw,Dimiter S Dimitrov,Wei Li,Kristopher R Bosse
View all publications
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