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AB288422

Anti-CD35+CD21 antibody [7G6]

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(1 Publication)

Rabbit Recombinant Monoclonal CD21 antibody. Suitable for Flow Cyt, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Cr2.

View Alternative Names

CD21, Complement receptor type 2, Cr2, Complement C3d receptor

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35+CD21 antibody [7G6] (AB288422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35+CD21 antibody [7G6] (AB288422)

Immunohistochemical staining of rat stomach using anti-Complement receptors 1&2 antibody ab288422.

Formalin fixed rat stomach slices were were stained with ab288422 at 3 μg/ml.

Flow Cytometry - Anti-CD35+CD21 antibody [7G6] (AB288422)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD35+CD21 antibody [7G6] (AB288422)

Flow-cytometry using anti-complement receptors 1 & 2 antibody 7G6 (ab288422)

Mouse lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 7G6 (ab288422, panel B) at a concentration of 1 μg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

7G6

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat

Applications

Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Cr2.

P19070

Specificity

Recognises an epitope shared by 145-150kDa and 190kDa complement receptor proteins, originally designated CR2 (CD21) and CR1 (CD35).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.
See full target information Cr2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:104059 PubMed40892539

2025

Protocol for rapid 5-plex 3D imaging and single-cell analysis of immune responses in whole murine lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Victoire Boulat,Alessandro Ciccarelli,Miu Shing Hung,Lingling Zhang,Anita Grigoriadis,Dinis Pedro Calado
View all publications
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Product promise

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