Anti-CD36 antibody [EPR22509-40]
- RabMAb
- Recombinant
- 20ul selling size
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(42 Publications)
Anti-CD36 antibody [EPR22509-40] (ab252922) is a rabbit monoclonal antibody detecting CD36 in Western Blot, Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
View Alternative Names
CD36, GP3B, GP4, Platelet glycoprotein 4, Fatty acid translocase, Glycoprotein IIIb, Leukocyte differentiation antigen CD36, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, Thrombospondin receptor, FAT, GPIIIB, GPIV
- Flow Cyt
Unknown
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (AB252922)
Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (AB252922)
Immunohistochemical analysis of [paraffin-embedded human spleen tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human spleen (PMID : 30047927,22777768) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (AB252922)
Immunohistochemical analysis of paraffin-embedded human hepatocellular tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human hepatocellular carcinoma (PMID : 30047927,22777768) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-CD36 antibody [EPR22509-40] (AB252922)
Immunofluorescent analysis of 80% Methanol fixed 0.1% TritonX-100 permeabilised U937 (Human histiocytic lymphoma monocyte) cells labelling CD36 with ab252922 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing membranous staining in U937 cells (Human histiocytic lymphoma monocyte) is observed. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain Alpha Tubulin at 1/200 dilution.The Nuclear counterstain is DAPI . Secondary antibody only control : Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (AB252922)
Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach. (PMID : 30047927,22777768) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
- WB
Unknown
Western blot - Anti-CD36 antibody [EPR22509-40] (AB252922)
Blocking and dilution buffer : 5% NFDM/TBST.
Negative control : Ramos, Jurkat (PMID : 27956597,10744776)
The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID : 8798390,15677505,20637247, 8631821).
All lanes:
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution
Lane 1:
U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg
Lane 2:
Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Lane 3:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 4:
Rat spleen tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 70-110 kDa
false
Exposure time: 8s
- WB
Unknown
Western blot - Anti-CD36 antibody [EPR22509-40] (AB252922)
Blocking and dilution buffer : 5% NFDM/TBST.
The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID : 8798390,15677505,20637247).
All lanes:
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922) at 1000 Cells
Lane 1:
Human placenta tissue lysate at 20 µg
Lane 2:
Human lung tissue lysate at 20 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution
Predicted band size: 53 kDa
false
Exposure time: 3s
- WB
Lab
Western blot - Anti-CD36 antibody [EPR22509-40] (AB252922)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID : 8798390, 15677505, 20637247, 8631821).
Low expression : brain (PMID : 27646002 )
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes:
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution
Lane 1:
Mouse white fat tissue lysate at 40 µg
Lane 2:
Mouse brown fat tissue lysate at 40 µg
Lane 3:
Mouse spleen tissue lysate at 40 µg
Lane 4:
Mouse brain tissue lysate at 40 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa,100 kDa
true
Exposure time: 81s
- WB
Lab
Western blot - Anti-CD36 antibody [EPR22509-40] (AB252922)
Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM /TBST
CD36 expression is low in the normal brain (PMID : 27646002)
All lanes:
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution
Lane 1:
Rat spleen tissue lysate at 20 µg
Lane 2:
Rat brain tissue lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 70-110 kDa
false
Exposure time: 180s
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (AB252922)
Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control : Ramos (PMID : 10744776).
Related conjugates and formulations (2)
-
Anti-CD36 antibody [EPR22509-40] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-CD36 antibody [EPR22509-40]
Reactivity data
Product details
Anti-CD36 antibody [EPR22509-40] (ab252922) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-CD36 antibody [EPR22509-40] (ab252922) has been cited over 12 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD36 antibody [EPR22509-40] (ab252922) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD36 antibody [EPR22509-40] (ab252922) specifically detects CD36 (UniProt ID: P16671; Molecular weight: 53kDa) and is sold in 100 µL and 1 mL selling sizes.
Conjugation-ready, carrier free format available for antibody clone EPR22509-40 - ab255331.
Antibody clone EPR22509-40 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (ab320068).
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
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Shipped at conditions
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Storage information
Product protocols
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Target data
Publications (42)
Recent publications for all applications. Explore the full list and refine your search
Cell death & disease 16:579 PubMed40744915
2025
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Scientific reports 15:27344 PubMed40717126
2025
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BMJ open diabetes research & care 13: PubMed40716951
2025
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Food science & nutrition 13:e70599 PubMed40654536
2025
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Nature communications 16:5568 PubMed40593524
2025
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Frontiers in immunology 16:1577234 PubMed40475787
2025
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Molecular cancer 24:157 PubMed40442751
2025
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CNS neuroscience & therapeutics 31:e70413 PubMed40384564
2025
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International journal of molecular sciences 26: PubMed40331957
2025
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Pharmaceutics 17: PubMed40284439
2025
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com