AB252923

Anti-CD36 antibody [EPR22512-58]

Name: Anti-CD36 antibody [EPR22512-58] (ab252923) | Abcam
Brand: Abcam Limited
SKU: AB252923
Price: 380 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(17 Publications)

Anti-CD36 antibody [EPR22512-58] (ab252923) is a rabbit monoclonal antibody detecting CD36 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD36, GP3B, GP4, Platelet glycoprotein 4, Fatty acid translocase, Glycoprotein IIIb, Leukocyte differentiation antigen CD36, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, Thrombospondin receptor, FAT, GPIIIB, GPIV

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (AB252923)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (AB252923)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD36 with ab252923 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human liver (PMID : 30047927, 22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (AB252923)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD36 with ab252923 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of mouse spleen (PMID : 30047927, 22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (AB252923)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252923 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach (PMID : 30047927, 22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Western blot - Anti-CD36 antibody [EPR22512-58] (AB252923)

Blocking and dilution buffer : 5% NFDM/TBST.

Negative control : Ramos, Jurkat (PMID : 27956597, 10744776).

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID : 8798390,15677505,20637247,
8631821).

All lanes:

Western blot - Anti-CD36 antibody [EPR22512-58] (ab252923) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 100000 Cells

Predicted band size: 53 kDa

Observed band size: 70-110 kDa

false

Exposure time: 58s

Unknown

Western blot - Anti-CD36 antibody [EPR22512-58] (AB252923)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 5.5 seconds; Lane 2 : 15 seconds; Lane 3 : 48 seconds.

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID : 8798390,15677505,20637247).

All lanes:

Western blot - Anti-CD36 antibody [EPR22512-58] (ab252923) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human breast cancer tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 53 kDa

Observed band size: 70-110 kDa

false

Carrier free

Anti-CD36 antibody [EPR22512-58] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22512-58

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation are based on the IHC results. We do not guarantee WB for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-CD36 antibody [EPR22512-58] (ab252923) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of CD36?
Anti-CD36 [EPR22512-58] (ab252923) specifically detects a band for CD36 (UniProt: P16671) at a molecular weight of 53kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR22512-58] also available for your convenience: ab252923, Carrier free - ab255332

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional glycoprotein that acts as a receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed : 18353783, PubMed : 21395585, PubMed : 21610069). Mechanistically, palmitoylated CD36 captures fatty acids on the cell surface, the binding of fatty acids activates downstream kinase LYN, which phosphorylates the palmitoyltransferase ZDHHC5 and inactivates it, resulting in the subsequent depalmitoylation of CD36, a step needed to initiate the endocytic process and delivery of fatty acids into the cell (PubMed : 32958780). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed : 18753675). Involved in oral fat perception and preferences (PubMed : 22240721, PubMed : 25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thrombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting together with THBS1 (By similarity). As a coreceptor for TLR4 : TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4 : TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed : 20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2 : TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed : 16880211).. (Microbial infection) Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling.

See full target information CD36

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113405 PubMed40978144

2025

Histone demethylase LSD1 regulates lipid homeostasis during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Gaurav Kumar Lohia,Awantika Shah,Kithiganahalli Narayanaswamy Balaji

Cell death & disease 16:579 PubMed40744915

2025

PRMT1-mediated methylation of UBE2m promoting calcium oxalate crystal-induced kidney injury by inhibiting fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhui Yuan,Zehua Ye,Shuqin Mei,Miao Zhang,Ming Wu,Fangyou Lin,Weimin Yu,Wei Li,Xiangjun Zhou,Fan Cheng

Biology of sex differences 16:43 PubMed40524248

2025

Gestational saccharin consumption disrupts gut-brain axis glucose homeostasis control in adolescent offspring rats in a sex-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Beatriz Pacheco-Sánchez,Sonia Melgar-Locatelli,Raquel López-Merchán,María José Benítez-Marín,Marta Blasco-Alonso,Ernesto González-Mesa,Rubén Tovar,Pablo Rubio,Juan Suárez,Carlos Sanjuan,Fernando Rodríguez de Fonseca,Francisco Alén,Marialuisa de Ceglia,Patricia Rivera

International journal of medical sciences 22:1223-1236 PubMed40027179

2025

CD36 knockdown attenuates pressure overload-induced cardiac injury by preventing lipotoxicity and improving myocardial energy metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Geng,Xiaoliang Zhang,Ying Wang,Dong Guo,Panpan Liu,Siying Pu,Xue Yang,Qi Liang,Pan Chang,Tao Li,Lang Hu,Yanjie Guo

Nature communications 16:613 PubMed39800728

2025

Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Geng,Xiaoliang Zhang,Yanjie Guo,He Wen,Dong Guo,Qi Liang,Siying Pu,Ying Wang,Mingchuan Liu,Zhelong Li,Wei Hu,Xue Yang,Pan Chang,Lang Hu,Yan Li

Heliyon 10:e40806 PubMed39691192

2024

CB2R activation enhances tumor-associated macrophages-mediated phagocytosis of glioma cell.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Lu,Xuezhu Chen,Yang Yang,Junlong Li

International journal of molecular sciences 25: PubMed39125901

2024

Propagermanium as a Novel Therapeutic Approach for the Treatment of Endothelial Dysfunction in Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Azul,Adriana Leandro,Raquel Seiça,Cristina M Sena

Journal for immunotherapy of cancer 12: PubMed39067872

2024

POSTN cancer-associated fibroblasts determine the efficacy of immunotherapy in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Yuan Liang,Zheng Liu,Rui Zhang,Jiashuo Chao,Mingming Wang,Mu Liu,Lei Qiao,Zhengfeng Xuan,Haitao Zhao,Ling Lu

Cellular and molecular gastroenterology and hepatology 17:1039-1061 PubMed38467191

2024

Endothelial Slc35a1 Deficiency Causes Loss of LSEC Identity and Exacerbates Neonatal Lipid Deposition in the Liver in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zuo,Fei Yang,Lulu Huang,Jingjing Han,Tianyi Li,Zhenni Ma,Lijuan Cao,Yun Li,Xia Bai,Miao Jiang,Yang He,Lijun Xia

Food & function 15:2679-2692 PubMed38375746

2024

Limonin alleviates high-fat diet-induced dyslipidemia by regulating the intestinal barrier the microbiota-related ILC3-IL22-IL22R pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wangling Wu,Yingying Pan,Tianyan Zheng,Haoyi Sun,Xia Li,Haiyan Zhu,Zheng Wang,Xin Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD36 antibody [EPR22512-58]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (AB252923)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (AB252923)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (AB252923)

Western blot - Anti-CD36 antibody [EPR22512-58] (AB252923)

Western blot - Anti-CD36 antibody [EPR22512-58] (AB252923)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (17)

Product promise