Anti-CD36 antibody [EPR6573]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (AB133625)

ab133625 staining CD36 in paraffin embedded Human Hepatocellular cancer tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17μg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining on endothelial cells in human hepatocellular cancer.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (AB133625)

ab133625 staining CD36 in paraffin embedded Human cardiac muscle tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17μg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining mainly on endothelial cells in human cardiac muscle.

• WB

Supplier Data

Western blot - Anti-CD36 antibody [EPR6573] (AB133625)

This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133625 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

Lane 1:

Human Heart Tissue Lysate at 20 µg

Lane 2:

Human Adipose Tissue Lysate at 20 µg

Lane 3:

Human Platelet Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 53 kDa

Observed band size: 88 kDa

false

• WB

Supplier Data

Western blot - Anti-CD36 antibody [EPR6573] (AB133625)

The expression level of CD36 varies in different samples, and it could be upregulated by treatments such as PMA and Porphyromonas gingivalis (PMID : 8576181 and 27234131).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

We are unsure as to the identity of the 130kda band. Based on the data we currently have, we tend to believe that it is a nonspecific band.

All lanes:

Western blot - Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

Lane 1:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA (Phorbol-12-myristate-13-acetate) for 72 hours whole cell lysates at 20 µg

Lane 3:

Human adipose lysates at 20 µg

Lane 4:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 78 kDa

false

Exposure time: 50s

• WB

Lab

Western blot - Anti-CD36 antibody [EPR6573] (AB133625)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

All lanes:

HEK293 (human embryonic kidney epithelial cell) transfected with His-tagged human CD36 (30aa-439aa) expression vector, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 74 kDa

false

Exposure time: 3s