Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (AB221605)

ab133625 staining CD36 in paraffin embedded Human cardiac muscle tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining mainly on endothelial cells in human cardiac muscle.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (AB221605)

ab133625 staining CD36 in paraffin embedded Human Hepatocellular cancer tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining on endothelial cells in human hepatocellular cancer.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

• WB

Lab

Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (AB221605)

This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133625 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

All lanes:

Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605) at 1/1000 dilution

Lane 1:

Human Heart Tissue Lysate at 20 µg

Lane 2:

Human Adipose Tissue Lysate at 20 µg

Lanes 3 - 4:

Mouse Adipose Tissue Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 53 kDa

Observed band size: 88 kDa

true

Exposure time: 2min

• WB

Supplier Data

Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (AB221605)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

The expression level of CD36 varies in different samples, and it could be upregulated by treatments such as PMA and Porphyromonas gingivalis (PMID : 8576181 and 27234131).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

We are unsure as to the identity of the 130kda band. Based on the data we currently have, we tend to believe that it is a nonspecific band.

All lanes:

Western blot - Anti-CD36 antibody [EPR6573] (<a href='/en-us/products/primary-antibodies/cd36-antibody-epr6573-ab133625'>ab133625</a>) at 1/1000 dilution

Lane 1:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA (Phorbol-12-myristate-13-acetate) for 72 hours whole cell lysates at 20 µg

Lane 3:

Human adipose lysates at 20 µg

Lane 4:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 78 kDa

false

Exposure time: 50s

• WB

Lab

Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (AB221605)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD36 antibody [EPR6573] (<a href='/en-us/products/primary-antibodies/cd36-antibody-epr6573-ab133625'>ab133625</a>) at 1/1000 dilution

All lanes:

HEK293 (human embryonic kidney epithelial cell) transfected with His-tagged human CD36 (30aa-439aa) expression vector, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 74 kDa

false

Exposure time: 3s