Rabbit Polyclonal CD37 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD37 aa 100-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CD37 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P11049
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:22624718). Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B (PubMed:22624718). Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT (By similarity). Plays also a role in the migration of dendritic cells and neutrophils to draining lymph nodes, as well as in their integrin-mediated adhesion (By similarity). Negatively regulates IL-6 responses through direct interaction with SOCS3 thereby preventing constitutive IL-6 signaling (PubMed:26784544). Alternatively, inhibition of IL-6 signaling can also occur via interaction and stabilization of DECTIN1/CLEC7A at the cell membrane to inhibit its ability to promote the production of IL-6 (PubMed:17182550).
Alternative names
CD37, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26, Tspan-26
Recommended products
Rabbit Polyclonal CD37 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD37 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CD37 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link P11049
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody (ab251820)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD37 with ab251820 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody (ab251820)
Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD37 with ab251820 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody (ab251820)
Formalin-fixed, paraffin-embedded human skeletal muscle tissue show absence of immunoreactivity for CD37 when incubated with ab251820 at a 1/200 dilution (negative control).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody (ab251820)
Formalin-fixed, paraffin-embedded human cerebral cortex tissue show absence of immunoreactivity for CD37 when incubated with ab251820 at a 1/200 dilution (negative control).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody (ab251820)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD37 with Anti-CD37 antibody [RM1306] ab323574 at 1/300 (1.68 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human spleen.
The image on (A) Anti-CD37 antibody [RM1306] ab323574 is applied with Anti-CD37 antibody at 1/300 dilution and (B) ab251820 is applied with Anti-CD37 antibody at 1/50 dilution.
The section was incubated with Anti-CD37 antibody [RM1306] ab323574 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody (ab251820)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD37 with Anti-CD37 antibody [RM1306] ab323574 at 1/300 (1.68 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil.
The image on (A) Anti-CD37 antibody [RM1306] ab323574 is applied with Anti-CD37 antibody at 1/300 dilution and (B) ab251820 is applied with Anti-CD37 antibody at 1/50 dilution.
The section was incubated with Anti-CD37 antibody [RM1306] ab323574 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
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