Anti-CD37 antibody [EPR25397-136] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448)

This data was developed using ab288439, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Raji cells labelling CD37 with ab288439 at 1/50 (9.86 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in Raji cell line. Negative control : MCF7 (PMID : 33652767. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448)

This data was developed using ab288439, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD37 with ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Positive staining was found in human normal tonsil (PMID : 25934707). The section was incubated with ab288439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• Flow Cyt

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Flow Cytometry - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448)

This data was developed using ab288439, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD37 with ab288439 at 1/50 dilution (1ug)/ red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : MCF7 (PMID : 33652767). Gated on viable cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448)

This data was developed using ab288439, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling CD37 with ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Positive staining was found in human normal spleen (PMID : 25934707). The section was incubated with ab288439 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND ^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448)

This data was developed using ab288439, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labelling CD37 with ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Positive staining was found in human Hodgkin's lymphoma. The section was incubated with ab288439 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448)

This data was developed using ab288439, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling CD37 with ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Negative control : No staining was found on human skeletal muscle. The section was incubated with ab288439 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins