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Rabbit Recombinant Monoclonal CD37 antibody. Carrier free. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples.

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Images

Flow Cytometry - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (AB288448), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:22624718). Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B (PubMed:22624718). Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT (By similarity). Plays also a role in the migration of dendritic cells and neutrophils to draining lymph nodes, as well as in their integrin-mediated adhesion (By similarity). Negatively regulates IL-6 responses through direct interaction with SOCS3 thereby preventing constitutive IL-6 signaling (PubMed:26784544). Alternatively, inhibition of IL-6 signaling can also occur via interaction and stabilization of DECTIN1/CLEC7A at the cell membrane to inhibit its ability to promote the production of IL-6 (PubMed:17182550).

Alternative names

CD37, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26, Tspan-26

Recommended products

Rabbit Recombinant Monoclonal CD37 antibody. Carrier free. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25397-136
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448), expandable thumbnail

    Flow Cytometry - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448)

    This data was developed using Anti-CD37 antibody [EPR25397-136] ab288439, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD37 with Anti-CD37 antibody [EPR25397-136] ab288439 at 1/50 dilution (1ug)/ red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: MCF7 (PMID: 33652767). Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448)

    This data was developed using Anti-CD37 antibody [EPR25397-136] ab288439, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Raji cells labelling CD37 with Anti-CD37 antibody [EPR25397-136] ab288439 at 1/50 (9.86 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in Raji cell line. Negative control: MCF7 (PMID: 33652767. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448)

    This data was developed using Anti-CD37 antibody [EPR25397-136] ab288439, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling CD37 with Anti-CD37 antibody [EPR25397-136] ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Negative control: No staining was found on human skeletal muscle. The section was incubated with Anti-CD37 antibody [EPR25397-136] ab288439 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448)

    This data was developed using Anti-CD37 antibody [EPR25397-136] ab288439, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labelling CD37 with Anti-CD37 antibody [EPR25397-136] ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Positive staining was found in human Hodgkin's lymphoma. The section was incubated with Anti-CD37 antibody [EPR25397-136] ab288439 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448)

    This data was developed using Anti-CD37 antibody [EPR25397-136] ab288439, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling CD37 with Anti-CD37 antibody [EPR25397-136] ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Positive staining was found in human normal spleen (PMID:25934707). The section was incubated with Anti-CD37 antibody [EPR25397-136] ab288439 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND ® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-136] - BSA and Azide free (ab288448)

    This data was developed using Anti-CD37 antibody [EPR25397-136] ab288439, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD37 with Anti-CD37 antibody [EPR25397-136] ab288439 at 1/500 (0.986 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Positive staining was found in human normal tonsil (PMID:25934707). The section was incubated with Anti-CD37 antibody [EPR25397-136] ab288439 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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