Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)

Rabbit Recombinant Monoclonal CD37 antibody. Carrier free. Suitable for IP, Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (AB300412), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt	ICC/IF	IHC-P	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Target data

See full target information CD37_HUMAN

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:22624718). Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B (PubMed:22624718). Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT (By similarity). Plays also a role in the migration of dendritic cells and neutrophils to draining lymph nodes, as well as in their integrin-mediated adhesion (By similarity). Negatively regulates IL-6 responses through direct interaction with SOCS3 thereby preventing constitutive IL-6 signaling (PubMed:26784544). Alternatively, inhibition of IL-6 signaling can also occur via interaction and stabilization of DECTIN1/CLEC7A at the cell membrane to inhibit its ability to promote the production of IL-6 (PubMed:17182550).

Alternative names

CD37, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26, Tspan-26

Recommended products

Rabbit Recombinant Monoclonal CD37 antibody. Carrier free. Suitable for IP, Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR25397-149
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Flow Cytometry (Intracellular) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control. Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Cells were stained with rabbit IgG or Anti-CD37 antibody [EPR25397-149] ab300400. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Immunoprecipitation - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
This data was developed using Anti-CD37 antibody [EPR25397-149] ab300400, the same antibody clone in a different buffer formulation.CD37 was immunoprecipitated from 0.35 mg Human tonsil tisseu lysate 10 ug with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD37 antibody [EPR25397-149] ab300400 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human tonsil tisseu lysate 10 ug
Lane 2: abAB300400 IP in Human tonsil tisseu lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD37 antibody [EPR25397-149] ab300400 in Human tonsil tisseu lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds
All lanes: Immunoprecipitation - Anti-CD37 antibody [EPR25397-149] (Anti-CD37 antibody [EPR25397-149] ab300400) at 1/1000 dilution
All lanes: Human tonsil tissue lysate at 10 µg
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s
Flow Cytometry (Intracellular) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control: MCF7 (PMID: 33652767).
Flow Cytometry (Intracellular) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
This data was developed using Anti-CD37 antibody [EPR25397-149] ab300400, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 (1.086 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in Raji cell lineNegative control: MCF7 (PMID: 33652767? is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Western blot - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
This data was developed using Anti-CD37 antibody [EPR25397-149] ab300400, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-CD37 antibody [EPR25397-149] (Anti-CD37 antibody [EPR25397-149] ab300400) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate
Lane 2: Human skeletal muscle tissue lysate
Lane 3: Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
This data was developed using Anti-CD37 antibody [EPR25397-149] ab300400, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control: No staining was found on human skeletal muscle. The section was incubated with Anti-CD37 antibody [EPR25397-149] ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
This data was developed using Anti-CD37 antibody [EPR25397-149] ab300400, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymp tissue labeling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining was found in human Hodgkin's lymphoma. The section was incubated with Anti-CD37 antibody [EPR25397-149] ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
This data was developed using Anti-CD37 antibody [EPR25397-149] ab300400, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining was found in human spleen (PMID: 25934707). The section was incubated with Anti-CD37 antibody [EPR25397-149] ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR25397-149] (BSA and Azide free) (ab300412)
This data was developed using Anti-CD37 antibody [EPR25397-149] ab300400, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD37 with Anti-CD37 antibody [EPR25397-149] ab300400 at 1/500 (0.986 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining was found in human tonsil (PMID: 25934707). The section was incubated with Anti-CD37 antibody [EPR25397-149] ab300400 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins