Anti-CD37 antibody [EPR28769-31] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD37 with ab315346 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse cardiac muscle. The section was incubated with ab315346 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse Burkitt's lymphoma tissue labeling CD37 with ab315346 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse Burkitt's lymphoma. The section was incubated with ab315346 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD37 with ab315346 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spleen (PMID : 19282981). The section was incubated with ab315346 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes cells labelling CD37 with ab315346 at 1/500 (1.026 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing membranous staining in subsets of mouse splenocytes, which is co-localized with anti-CD45R. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Anti-human/mouse CD45R/B220 rat monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling CD37 with ab315346 at 1/50 dilution (1 ug, Right) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells are co-stained with CD45R/B220 conjugated to Alexa Fluor®647.

• IP

Supplier Data

Immunoprecipitation - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

CD37 was immunoprecipitated from 0.35 mg Mouse spleen whole cell lysate with ab315346 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315346 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse spleen whole cell lysate

Lane 2 : ab315346 IP in Mouse spleen whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315346 in mouse spleen whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD37 antibody [EPR28769-31] (<a href='/en-us/products/primary-antibodies/cd37-antibody-epr28769-31-ab315346'>ab315346</a>) at 1/30 dilution

All lanes:

Mouse spleen whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Mouse heart.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD37 antibody [EPR28769-31] (<a href='/en-us/products/primary-antibodies/cd37-antibody-epr28769-31-ab315346'>ab315346</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40-60 kDa,36 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347)

This data was developed using ab315346, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD37 antibody [EPR28769-31] (<a href='/en-us/products/primary-antibodies/cd37-antibody-epr28769-31-ab315346'>ab315346</a>) at 1/1000 dilution

All lanes:

WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40-60 kDa,36 kDa

false

Exposure time: 37s