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Rabbit Recombinant Monoclonal CD37 antibody. Carrier free. Suitable for WB, IP, IHC-P, Flow Cyt, ICC/IF and reacts with Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347), expandable thumbnail
  • Western blot - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (AB315347), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PFlow CytICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:10891477). Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B (By similarity). Plays an essential role for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT (PubMed:23150881). Plays also a role in the migration of dendritic cells and neutrophils to draining lymph nodes, as well as in their integrin-mediated adhesion (PubMed:23420539, PubMed:26566675). Negatively regulates IL-6 responses through direct interaction with SOCS3 thereby preventing constitutive IL-6 signaling (By similarity). Alternatively, inhibition of IL-6 signaling can also occur via interaction and stabilization of DECTIN1/CLEC7A at the cell membrane to inhibit its ability to promote the production of IL-6 (By similarity).

Alternative names

CD37, Leukocyte antigen CD37, Cd37

Recommended products

Rabbit Recombinant Monoclonal CD37 antibody. Carrier free. Suitable for WB, IP, IHC-P, Flow Cyt, ICC/IF and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28769-31
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315347 is the carrier-free version of Anti-CD37 antibody [EPR28769-31] ab315346.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes cells labelling CD37 with Anti-CD37 antibody [EPR28769-31] ab315346 at 1/500 (1.026 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing membranous staining in subsets of mouse splenocytes, which is co-localized with anti-CD45R. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-human/mouse CD45R/B220 rat monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD37 with Anti-CD37 antibody [EPR28769-31] ab315346 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse cardiac muscle. The section was incubated with Anti-CD37 antibody [EPR28769-31] ab315346 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse Burkitt's lymphoma tissue labeling CD37 with Anti-CD37 antibody [EPR28769-31] ab315346 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse Burkitt's lymphoma. The section was incubated with Anti-CD37 antibody [EPR28769-31] ab315346 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD37 with Anti-CD37 antibody [EPR28769-31] ab315346 at 1/2000 (0.257 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen (PMID: 19282981). The section was incubated with Anti-CD37 antibody [EPR28769-31] ab315346 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Western blot - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CD37 antibody [EPR28769-31] (Anti-CD37 antibody [EPR28769-31] ab315346) at 1/1000 dilution

    All lanes: WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40-60 kDa, 36 kDa

    Exposure time: 37s

  • Western blot - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Western blot - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Mouse heart.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CD37 antibody [EPR28769-31] (Anti-CD37 antibody [EPR28769-31] ab315346) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40-60 kDa, 36 kDa

    Exposure time: 37s

  • Flow Cytometry - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Flow Cytometry - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse splenocytes cells labelling CD37 with Anti-CD37 antibody [EPR28769-31] ab315346 at 1/50 dilution (1 ug, Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells. Cells are co-stained with CD45R/B220 conjugated to Alexa Fluor®647.

  • Immunoprecipitation - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347), expandable thumbnail

    Immunoprecipitation - Anti-CD37 antibody [EPR28769-31] - BSA and Azide free (ab315347)

    This data was developed using Anti-CD37 antibody [EPR28769-31] ab315346, the same antibody clone in a different buffer formulation.

    CD37 was immunoprecipitated from 0.35 mg Mouse spleen whole cell lysate with Anti-CD37 antibody [EPR28769-31] ab315346 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD37 antibody [EPR28769-31] ab315346 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen whole cell lysate

    Lane 2: Anti-CD37 antibody [EPR28769-31] ab315346 IP in Mouse spleen whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD37 antibody [EPR28769-31] ab315346 in mouse spleen whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CD37 antibody [EPR28769-31] (Anti-CD37 antibody [EPR28769-31] ab315346) at 1/30 dilution

    All lanes: Mouse spleen whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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