Anti-CD38 antibody [5C5C3] - Extracellular domain

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [5C5C3] - Extracellular domain (AB204940)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD38 with ab204940 at 1/200 dilution with DAB staining.

• WB

Supplier Data

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (AB204940)

All lanes:

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (ab204940) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

LNcap cell lysate

Predicted band size: 34 kDa

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• WB

Lab

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (AB204940)

Anti-CD38 antibody [5C5C3] - Extracellular domain staining at 1/500 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (ab108198) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab204940 was shown to bind specifically to CD38. A band was observed at 42 kDa in wild-type A549 cell lysates with no signal observed at this size in CD38 knockout cell line. To generate this image, wild-type and CD38 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (ab204940) at 1/500 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human CD38 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cd38-knockout-a549-cell-line-ab289025'>ab289025</a>)

Lane 2:

CD38 knockout A549 cell lysate at 20 µg

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

HCT 116 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 42 kDa

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• WB

Supplier Data

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (AB204940)

All lanes:

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (ab204940) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

CD38 (aa43-300)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (AB204940)

Expected MWt is 56.1 kDa.

All lanes:

Western blot - Anti-CD38 antibody [5C5C3] - Extracellular domain (ab204940) at 1/500 dilution

All lanes:

Recombinant Human CD38 (aa43-300) protein

Predicted band size: 34 kDa

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