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Rabbit Recombinant Monoclonal CD38 antibody. Suitable for ICC, IHC-P, Flow Cyt, IP, WB and reacts with Human samples.

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Images

Western blot - Anti-CD38 antibody [BLR123H] - BSA free (AB314061), expandable thumbnail
  • Immunoprecipitation - Anti-CD38 antibody [BLR123H] - BSA free (AB314061), expandable thumbnail
  • Flow Cytometry - Anti-CD38 antibody [BLR123H] - BSA free (AB314061), expandable thumbnail
  • Immunocytochemistry - Anti-CD38 antibody [BLR123H] - BSA free (AB314061), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link P28907

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIHC-PFlow CytIPWB
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed:7961800, PubMed:8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed:16690024). Has cADPR hydrolase activity (PubMed:7961800, PubMed:8253715).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD38 antibody. Suitable for ICC, IHC-P, Flow Cyt, IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link P28907
Clone number
BLR123H
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-CD38 antibody [BLR123H] - BSA free (ab314061), expandable thumbnail

    Western blot - Anti-CD38 antibody [BLR123H] - BSA free (ab314061)

    Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, MOLT-4, RPMI-8226, CCRF-CEM, A-549, Ramos, Raji, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody

    All lanes: Western blot - Anti-CD38 antibody [BLR123H] - BSA free (ab314061) at 1/1000 dilution

    Lane 1: HeLa Whole cell lysate at 10 µg

    Lane 2: HEK293T Whole cell lysate at 10 µg

    Lane 3: Jurkat Whole cell lysate at 10 µg

    Lane 4: MOLT-4 Whole cell lysate at 10 µg

    Lane 5: RPMI-8226 Whole cell lysate at 10 µg

    Lane 6: CCRF-CEM Whole cell lysate at 10 µg

    Lane 7: A-549 Whole cell lysate at 10 µg

    Lane 8: Ramos Whole cell lysate at 10 µg

    Lane 9: Raji Whole cell lysate at 10 µg

    Lane 10: KG-1 Whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-CD38 antibody [BLR123H] - BSA free (ab314061), expandable thumbnail

    Immunoprecipitation - Anti-CD38 antibody [BLR123H] - BSA free (ab314061)

    Samples:Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (A700-123 lot 1) used for IP at 20 µl/mg lysate. CD38 was also immunoprecipitated by rabbit anti-CD38 antibody BL1874B-2A3. For blotting immunoprecipitated CD38, A700-123 was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.

  • Flow Cytometry - Anti-CD38 antibody [BLR123H] - BSA free (ab314061), expandable thumbnail

    Flow Cytometry - Anti-CD38 antibody [BLR123H] - BSA free (ab314061)

    Detection of human CD38 (shaded) in Ramos cells by flow cytometry.
    Antibody: Rabbit anti-CD38 recombinant monoclonal [BLR123H] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG

  • Immunocytochemistry - Anti-CD38 antibody [BLR123H] - BSA free (ab314061), expandable thumbnail

    Immunocytochemistry - Anti-CD38 antibody [BLR123H] - BSA free (ab314061)

    Detection of human CD38 in FFPE CCRF-CEM cells by immunocytochemistry. Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H]. Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (ab314061), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (ab314061)

    Detection of human CD38 in FFPE breast carcinoma by immunohistochemistry Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] . Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (ab314061), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (ab314061)

    Detection of human CD38 in FFPE tonsil by immunohistochemistry Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] . Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (ab314061), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (ab314061)

    Detection of human CD38 in FFPE colon carcinoma by immunohistochemistry. Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H]. Secondary:HRP-conjugated goat anti-rabbit IgG Substrate:DAB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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