Rabbit Recombinant Monoclonal CD38 antibody. Suitable for ICC, IHC-P, Flow Cyt, IP, WB and reacts with Human samples.
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
ICC | IHC-P | Flow Cyt | IP | WB | |
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Human | Tested | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed:7961800, PubMed:8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed:16690024). Has cADPR hydrolase activity (PubMed:7961800, PubMed:8253715).
CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1
Rabbit Recombinant Monoclonal CD38 antibody. Suitable for ICC, IHC-P, Flow Cyt, IP, WB and reacts with Human samples.
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, MOLT-4, RPMI-8226, CCRF-CEM, A-549, Ramos, Raji, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody
All lanes: Western blot - Anti-CD38 antibody [BLR123H] - BSA free (ab314061) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 10 µg
Lane 2: HEK293T Whole cell lysate at 10 µg
Lane 3: Jurkat Whole cell lysate at 10 µg
Lane 4: MOLT-4 Whole cell lysate at 10 µg
Lane 5: RPMI-8226 Whole cell lysate at 10 µg
Lane 6: CCRF-CEM Whole cell lysate at 10 µg
Lane 7: A-549 Whole cell lysate at 10 µg
Lane 8: Ramos Whole cell lysate at 10 µg
Lane 9: Raji Whole cell lysate at 10 µg
Lane 10: KG-1 Whole cell lysate at 10 µg
All lanes: HRP-conjugated goat anti-rabbit IgG
Samples:Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (A700-123 lot 1) used for IP at 20 µl/mg lysate. CD38 was also immunoprecipitated by rabbit anti-CD38 antibody BL1874B-2A3. For blotting immunoprecipitated CD38, A700-123 was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.
Detection of human CD38 (shaded) in Ramos cells by flow cytometry.
Antibody: Rabbit anti-CD38 recombinant monoclonal [BLR123H] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG
Detection of human CD38 in FFPE CCRF-CEM cells by immunocytochemistry. Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H]. Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.
Detection of human CD38 in FFPE breast carcinoma by immunohistochemistry Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] . Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.
Detection of human CD38 in FFPE tonsil by immunohistochemistry Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] . Secondary:HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.
Detection of human CD38 in FFPE colon carcinoma by immunohistochemistry. Antibody:Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H]. Secondary:HRP-conjugated goat anti-rabbit IgG Substrate:DAB.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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