AB314061

Anti-CD38 antibody [BLR123H] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal CD38 antibody. Suitable for ICC, IHC-P, Flow Cyt, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD38 aa 1 to C-terminus.

View Alternative Names

CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Detection of human CD38 in FFPE colon carcinoma by immunohistochemistry. Antibody : Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H]. Secondary : HRP-conjugated goat anti-rabbit IgG Substrate : DAB.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Detection of human CD38 in FFPE tonsil by immunohistochemistry Antibody : Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] . Secondary : HRP-conjugated goat anti-rabbit IgG . Substrate : DAB.

Flow Cytometry - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Flow Cyt

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Flow Cytometry - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Detection of human CD38 (shaded) in Ramos cells by flow cytometry. Antibody : Rabbit anti-CD38 recombinant monoclonal [BLR123H] or isotype control (unshaded). Secondary : DyLight® 488-conjugated goat anti-rabbit IgG

Supplier Data

Immunocytochemistry - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Detection of human CD38 in FFPE CCRF-CEM cells by immunocytochemistry. Antibody : Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H]. Secondary : HRP-conjugated goat anti-rabbit IgG . Substrate : DAB.

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Detection of human CD38 in FFPE breast carcinoma by immunohistochemistry Antibody : Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] . Secondary : HRP-conjugated goat anti-rabbit IgG . Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Samples : Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] (A700-123 lot 1) used for IP at 20 µl/mg lysate. CD38 was also immunoprecipitated by rabbit anti-CD38 antibody BL1874B-2A3. For blotting immunoprecipitated CD38, A700-123 was used at 1 : 1000. Chemiluminescence with an exposure time of 75 seconds.

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Western blot - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Samples : Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, MOLT-4, RPMI-8226, CCRF-CEM, A-549, Ramos, Raji, and KG-1 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-CD38 recombinant monoclonal antibody [BLR123H] used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-CD38 antibody [BLR123H] - BSA free (ab314061) at 1/1000 dilution

Lane 1:

HeLa Whole cell lysate at 10 µg

Lane 2:

HEK293T Whole cell lysate at 10 µg

Lane 3:

Jurkat Whole cell lysate at 10 µg

Lane 4:

MOLT-4 Whole cell lysate at 10 µg

Lane 5:

RPMI-8226 Whole cell lysate at 10 µg

Lane 6:

CCRF-CEM Whole cell lysate at 10 µg

Lane 7:

A-549 Whole cell lysate at 10 µg

Lane 8:

Ramos Whole cell lysate at 10 µg

Lane 9:

Raji Whole cell lysate at 10 µg

Lane 10:

KG-1 Whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR123H

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P, ICC, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD38 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P28907

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed : 7961800, PubMed : 8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed : 16690024). Has cADPR hydrolase activity (PubMed : 7961800, PubMed : 8253715).

See full target information CD38

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD38 antibody [BLR123H] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Flow Cytometry - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Immunocytochemistry - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Immunoprecipitation - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Western blot - Anti-CD38 antibody [BLR123H] - BSA free (AB314061)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise