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Rabbit Recombinant Monoclonal CD38 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 11 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343), expandable thumbnail
  • Western blot - Anti-CD38 antibody [EPR21079] (AB216343), expandable thumbnail
  • Western blot - Anti-CD38 antibody [EPR21079] (AB216343), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Mouse
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Synthesizes the second messengers cyclic ADP-ribose (cADPR) and nicotinate-adenine dinucleotide phosphate (NAADP), the former a second messenger for glucose-induced insulin secretion, the latter a Ca(2+) mobilizer (PubMed:11829748). Also has cADPR hydrolase activity (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD38 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 11 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR21079

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in endothelial cells of mouse kidney (PMID: 25078456). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the sinusoids of mouse liver (PMID: 17200192). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse spleen (PMID: 10360965). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Western blot - Anti-CD38 antibody [EPR21079] (ab216343)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The observed molecular mass is consistent with the literature (PMID: 7774637).

    All lanes: Western blot - Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution

    Lane 1: Mouse spleen lysate at 20 µg

    Lane 2: P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate at 20 µg

    Lane 3: Mouse lymph node lysate at 20 µg

    Lane 4: Mouse colon lysate at 20 µg

    Lane 5: Mouse thymus lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

  • Western blot - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Western blot - Anti-CD38 antibody [EPR21079] (ab216343)

    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD38-KO homozygous mice (Strain ID: T011481).

    All lanes: Western blot - Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution

    Lane 1: Wild-type mouse kidney tissue lysate (male)

    Lane 2: CD38 knockout mouse kidney tissue lysate (male)

    Lane 3: Wild-type mouse liver tissue lysate (male)

    Lane 4: CD38 knockout mouse liver tissue lysate (male)

    Lane 5: Wild-type mouse spleen tissue lysate (male)

    Lane 6: CD38 knockout spleen tissue lysate (male)

    Lane 7: Wild-type mouse kidney tissue lysate (female)

    Lane 8: CD38 knockout mouse kidney tissue lysate (female)

    Lane 9: Wild-type mouse liver tissue lysate (female)

    Lane 10: CD38 knockout mouse liver tissue lysate (female)

    Lane 11: Wild-type mouse spleen tissue lysate (female)

    Lane 12: CD38 knockout mouse spleen tissue lysate (female)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

    Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

    Immunohistochemical analysis of paraffin-embedded (A) Kidney tissue from wild-type C57BL/6JGpt mice (B) Kidney tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Kidney tissue from wild-type C57BL/6JGpt mice and no staining on (B) Kidney tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (ab216343)

    Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
    The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481).

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Product protocols

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