Anti-CD38 antibody [EPR21079]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in endothelial cells of mouse kidney (PMID : 25078456). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse spleen (PMID : 10360965). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343)

Immunohistochemical analysis of paraffin-embedded (A) Kidney tissue from wild-type C57BL/6JGpt mice (B) Kidney tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Kidney tissue from wild-type C57BL/6JGpt mice and no staining on (B) Kidney tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID : T011481).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the sinusoids of mouse liver (PMID : 17200192). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343)

Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID : T011481).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR21079] (AB216343)

Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID : T011481).

• WB

Supplier Data

Western blot - Anti-CD38 antibody [EPR21079] (AB216343)

Blocking/Dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The observed molecular mass is consistent with the literature (PMID : 7774637).

All lanes:

Western blot - Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 20 µg

Lane 2:

P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate at 20 µg

Lane 3:

Mouse lymph node lysate at 20 µg

Lane 4:

Mouse colon lysate at 20 µg

Lane 5:

Mouse thymus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 42 kDa

true

Exposure time: 3min

• WB

Lab

Western blot - Anti-CD38 antibody [EPR21079] (AB216343)

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD38-KO homozygous mice (Strain ID : T011481).

All lanes:

Western blot - Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution

Lane 1:

Wild-type mouse kidney tissue lysate (male)

Lane 2:

CD38 knockout mouse kidney tissue lysate (male)

Lane 3:

Wild-type mouse liver tissue lysate (male)

Lane 4:

CD38 knockout mouse liver tissue lysate (male)

Lane 5:

Wild-type mouse spleen tissue lysate (male)

Lane 6:

CD38 knockout spleen tissue lysate (male)

Lane 7:

Wild-type mouse kidney tissue lysate (female)

Lane 8:

CD38 knockout mouse kidney tissue lysate (female)

Lane 9:

Wild-type mouse liver tissue lysate (female)

Lane 10:

CD38 knockout mouse liver tissue lysate (female)

Lane 11:

Wild-type mouse spleen tissue lysate (female)

Lane 12:

CD38 knockout mouse spleen tissue lysate (female)

false