Rabbit Recombinant Monoclonal CD38 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 11 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | |
---|---|---|
Mouse | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Synthesizes the second messengers cyclic ADP-ribose (cADPR) and nicotinate-adenine dinucleotide phosphate (NAADP), the former a second messenger for glucose-induced insulin secretion, the latter a Ca(2+) mobilizer (PubMed:11829748). Also has cADPR hydrolase activity (By similarity).
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, I-19, NIM-R5 antigen, ADPRC 1, cADPR hydrolase 1, Cd38
Rabbit Recombinant Monoclonal CD38 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 11 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR21079
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in endothelial cells of mouse kidney (PMID: 25078456). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the sinusoids of mouse liver (PMID: 17200192). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD38 with ab216343 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse spleen (PMID: 10360965). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking/Dilution buffer: 5% NFDM/TBST.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument. The observed molecular mass is consistent with the literature (PMID: 7774637).
All lanes: Western blot - Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution
Lane 1: Mouse spleen lysate at 20 µg
Lane 2: P388D1 (mouse lymphoma monocyte; macrophage cell line) whole cell lysate at 20 µg
Lane 3: Mouse lymph node lysate at 20 µg
Lane 4: Mouse colon lysate at 20 µg
Lane 5: Mouse thymus lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 42 kDa
Exposure time: 3min
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD38-KO homozygous mice (Strain ID: T011481).
All lanes: Western blot - Anti-CD38 antibody [EPR21079] (ab216343) at 1/1000 dilution
Lane 1: Wild-type mouse kidney tissue lysate (male)
Lane 2: CD38 knockout mouse kidney tissue lysate (male)
Lane 3: Wild-type mouse liver tissue lysate (male)
Lane 4: CD38 knockout mouse liver tissue lysate (male)
Lane 5: Wild-type mouse spleen tissue lysate (male)
Lane 6: CD38 knockout spleen tissue lysate (male)
Lane 7: Wild-type mouse kidney tissue lysate (female)
Lane 8: CD38 knockout mouse kidney tissue lysate (female)
Lane 9: Wild-type mouse liver tissue lysate (female)
Lane 10: CD38 knockout mouse liver tissue lysate (female)
Lane 11: Wild-type mouse spleen tissue lysate (female)
Lane 12: CD38 knockout mouse spleen tissue lysate (female)
Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481).
Immunohistochemical analysis of paraffin-embedded (A) Kidney tissue from wild-type C57BL/6JGpt mice (B) Kidney tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Kidney tissue from wild-type C57BL/6JGpt mice and no staining on (B) Kidney tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481).
Immunohistochemical analysis of paraffin-embedded (A) Liver tissue from wild-type C57BL/6JGpt mice (B) Liver tissue from CD38 knockout mice staining with ab216343 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Liver tissue from wild-type C57BL/6JGpt mice and no staining on (B) Liver tissue from CD38 knockout mice. The section was incubated with ab216343 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND™ RX instrument
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Cd38-KO homozygous mice (Strain ID: T011481).
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