Anti-CD38 antibody [EPR4106]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

Tissue Microarrays stained for "Anti-CD38 antibody [EPR4106]" using "ab108403"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab108403 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• WB

Unknown

Western blot - Anti-CD38 antibody [EPR4106] (AB108403)

Buffer and concentration : 5% NFDM/TBST

Exposure time, Lane 1 : 20 seconds, Lane 2 : 30 seconds

Lane 1:

Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/200 dilution

Lane 2:

Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate at 20 µg

Lane 2:

Ramos (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

false

• WB

Unknown

Western blot - Anti-CD38 antibody [EPR4106] (AB108403)

Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/3000 dilution

All lanes:

Human lymph node lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 34 kDa

false

Exposure time: 80s

• WB

Unknown

Western blot - Anti-CD38 antibody [EPR4106] (AB108403)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution

Lane 1:

HuT-78 cell lysate at 20 µg

Lane 2:

Human fetal thymus tissue lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 45 kDa

false

• WB

Lab

Western blot - Anti-CD38 antibody [EPR4106] (AB108403)

Anti-CD38 antibody [EPR4106] staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108403 was shown to bind specifically to CD38. A band was observed at 42 kDa in wild-type A549 cell lysates with no signal observed at this size in CD38 knockout cell line. To generate this image, wild-type and CD38 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human CD38 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-cd38-knockout-a549-cell-line-ab289025'>ab289025</a>)

Lane 2:

CD38 knockout A549 cell lysate at 20 µg

Lane 3:

Daudi cell lysate at 20 µg

Lane 4:

HCT 116 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 42 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD38 antibody [EPR4106] (AB108403)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

• WB

CiteAb

Western blot - Anti-CD38 antibody [EPR4106] (AB108403)

CD38 western blot using anti-CD38 antibody [EPR4106] ab108403. Publication image and figure legend from Ning, L., Shan, G., et al., 2019, Biomed Res Int, PubMed 31179321.

ab108403 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108403 please see the product overview.

CD38 is increased in IBD patients. (a) Western blot analysis of CD38 expression in control and UC patients (N=3 per group). (b) Western blot analysis of CD38 expression in 3 matched pairs of inflamed (I) and noninflamed (NI) UC tissues. (c) Western blot analysis of CD38 expression in control and CD patients (N=3 per group). (d) Western blot analysis of CD38 expression in 3 matched pairs of inflamed (I) and noninflamed (NI) CD tissues. (e) Expression of CD38 by IHC (original magnification×100). (f) Immunofluorescence staining of DAPI (blue), CD38 (green), and F4/80 (red) in control, UC, and CD patients (N=3 per group) (original magnification, ×200). CD, Crohn's disease; UC, ulcerative colitis.

false