Anti-CD38 antibody [EPR4106] (ab108403)

Anti-CD38 antibody [EPR4106] (ab108403) is a rabbit monoclonal antibody detecting CD38 in Western Blot, IHC-P. Suitable for Human.

- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 10 publications

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt	WB	ICC/IF
Human	Tested	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500 - 1/1000	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes For unpurified use at 1/1000 - 1/10000.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Associated Products

Select an associated product type

15 products for Alternative Product

1 product for Alternative Version

Target data

See full target information CD38

Function

Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed:7961800, PubMed:8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed:16690024). Has cADPR hydrolase activity (PubMed:7961800, PubMed:8253715).

Alternative names

CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR4106
Purification technique: Affinity purification Protein A
Specificity: Not suitable for mouse and rat samples because antibody shows a non-specific cytoplasmic staining in almost all tested mouse and rat tissues.
Dissociation constant: 1.4 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-CD38 antibody [EPR4106] (ab108403) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of CD38?

Anti-CD38 [EPR4106] (ab108403) specifically detects a band for CD38 (UniProt: P28907) at a molecular weight of 34kDa.

Trusted by the scientific community

Anti-CD38 [EPR4106] (ab108403) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-CD38 antibody [EPR4106] (ab108403) has been confirmed by Western blot testing in CD38 Knockout A549 cells.

Other related products

We have a range of other formats of antibody clone [EPR4106] also available for your convenience:
ab108403, Carrier free - ab176886, Biotin - ab201498, Carrier free - ab226034, Alexa Fluor® 555 - ab279332, Alexa Fluor® 647 - ab279690, Alexa Fluor® 594 - ab280842, Alexa Fluor® 488 - ab323658, PE - ab323659

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
Buffer and concentration: 5% NFDM/TBST
Exposure time, Lane 1: 20 seconds, Lane 2: 30 seconds
Lane 1: Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/200 dilution
Lane 2: Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/1000 dilution
Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate at 20 µg
Lane 2: Ramos (Human Burkitt's lymphoma B lymphocyte), Whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/3000 dilution
All lanes: Human lymph node lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 34 kDa
Exposure time: 80s
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution
Lane 1: HuT-78 cell lysate at 20 µg
Lane 2: Human fetal thymus tissue lysate at 20 µg
Lane 3: Raji cell lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling CD38 with purified ab108403 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial adenocarcinoma tissue labelling CD38 with unpurified ab108403 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling CD38 with unpurified ab108403.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human colon tissue labelling CD38 with unpurified ab108403.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD38 antibody [EPR4106] (ab108403)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
Anti-CD38 antibody [EPR4106] staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108403 was shown to bind specifically to CD38. A band was observed at 42 kDa in wild-type A549 cell lysates with no signal observed at this size in CD38 knockout cell line. To generate this image, wild-type and CD38 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CD38 antibody [EPR4106] (ab108403) at 1/2000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: Western blot - Human CD38 knockout A549 cell line (Human CD38 knockout A549 cell line ab289025)
Lane 2: CD38 knockout A549 cell lysate at 20 µg
Lane 3: Daudi cell lysate at 20 µg
Lane 4: HCT 116 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (ab108403)
Tissue Microarrays stained for "Anti-CD38 antibody [EPR4106]" using "ab108403"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab108403 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-CD38 antibody [EPR4106] (ab108403)
CD38 western blot using anti-CD38 antibody [EPR4106] ab108403. Publication image and figure legend from Ning, L., Shan, G., et al., 2019, Biomed Res Int, PubMed 31179321.

ab108403 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108403 please see the product overview.
CD38 is increased in IBD patients. (a) Western blot analysis of CD38 expression in control and UC patients (N=3 per group). (b) Western blot analysis of CD38 expression in 3 matched pairs of inflamed (I) and noninflamed (NI) UC tissues. (c) Western blot analysis of CD38 expression in control and CD patients (N=3 per group). (d) Western blot analysis of CD38 expression in 3 matched pairs of inflamed (I) and noninflamed (NI) CD tissues. (e) Expression of CD38 by IHC (original magnification×100). (f) Immunofluorescence staining of DAPI (blue), CD38 (green), and F4/80 (red) in control, UC, and CD patients (N=3 per group) (original magnification, ×200). CD, Crohn's disease; UC, ulcerative colitis.