Mouse Recombinant Monoclonal CD38 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Flow Cyt | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.12 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5.6 µg/mL | Notes - |
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Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed:7961800, PubMed:8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed:16690024). Has cADPR hydrolase activity (PubMed:7961800, PubMed:8253715).
CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1
Mouse Recombinant Monoclonal CD38 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling CD38 with ab235118 at 1.12μg/ml (red) compared with a Mouse IgG, monoclonal - Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD38 with ab235118 at 5.6μg/ml, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Raji cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD38 with ab235118 at 1.12μg/ml compared with Mouse monoclonal IgG Isotype Control (left). Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human primary peripheral blood mononuclear cell) cells labeling CD38 with ab235118 at 5.6μg/ml, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in subsets of human PBMC. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at a 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab235118 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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