Rabbit Recombinant Monoclonal CD39 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications.
Images
![Western blot - Anti-CD39 antibody [EPR20461] (AB223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--western-blot-img182800.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD39 antibody [EPR20461] (AB223843)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (AB223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65540.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (AB223843)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (AB223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49730.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (AB223843)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (AB223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138907.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (AB223843)")
Publications
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- ImmunoHorizons 7:366-3792023SRF617 Is a Potent Inhibitor of CD39 with Immunomodulatory and Antitumor Properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael C Warren,Stephan Matissek,Matthew Rausch,Marisella Panduro,R J Hall,Austin Dulak,David Brennan,Sonia Das Yekkirala,Secil Koseoglu,Ricard Masia,Yu Yang,Navamallika Reddy,Robert Prenovitz,Jamie Strand,Tauqueer Zaidi,Erik Devereaux,Célia Jacoberger Foissac,John Stagg,Benjamin H Lee,Pamela Holland,Vito J Palombella,Andrew C LakePubMed 37219538
- Cancer immunology research 11:56-712022CD73 Inhibits cGAS-STING and Cooperates with CD39 to Promote Pancreatic Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCélia Jacoberger-Foissac,Isabelle Cousineau,Yacine Bareche,David Allard,Pavel Chrobak,Bertrand Allard,Sandra Pommey,Nouredin Messaoudi,Yannic McNicoll,Geneviève Soucy,Secil Koseoglu,Ricard Masia,Andrew C Lake,Heewon Seo,Christopher B Eeles,Neha Rohatgi,Simon C Robson,Simon Turcotte,Benjamin Haibe-Kains,John StaggPubMed 36409930
- Molecular human reproduction 27:2021Testicular adenosine acts as a pro-inflammatory molecule: role of testicular peritubular cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnika Missel,Lena Walenta,Katja Eubler,Nadine Mundt,Hanna Heikelä,Ulrich Pickl,Matthias Trottmann,Bastian Popper,Matti Poutanen,Leena Strauss,Frank-Michael Köhn,Lars Kunz,Marc Spehr,Artur MayerhoferPubMed 33993290
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Tested |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides (Probable) (PubMed:8529670, PubMed:8626624, PubMed:8955160, PubMed:8996251). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow (PubMed:8955160, PubMed:8996251).
Alternative names
CD39, ENTPD1, CD39, Ectonucleoside triphosphate diphosphohydrolase 1, ATP diphosphohydrolase, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, Nucleoside triphosphate diphosphohydrolase 1, ATP-DPH, ATPDase, Ecto-ATPDase 1, Ecto-ATPase 1, NTPDase1
Recommended products
Rabbit Recombinant Monoclonal CD39 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20461
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD39 antibody [EPR20461] (ab223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--western-blot-img182800.jpg "Western blot - Anti-CD39 antibody [EPR20461] (ab223843)")
Western blot - Anti-CD39 antibody [EPR20461] (ab223843)
Blocking buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD39 antibody [EPR20461] (ab223843) at 1/1000 dilution
Lane 1: Human kidney lysate at 15 µg
Lane 2: Human tonsil lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 78 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65540.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human pancreas (PMID: 18567823; PMID: 19308674). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49730.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-ab223843--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138907.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] (ab223843)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human tonsil (PMID: 27532024). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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