Rabbit Recombinant Monoclonal CD39 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65540.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49730.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140404.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427937.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Expected | Tested |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides (Probable) (PubMed:8529670, PubMed:8626624, PubMed:8955160, PubMed:8996251). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow (PubMed:8955160, PubMed:8996251).
Alternative names
CD39, ENTPD1, CD39, Ectonucleoside triphosphate diphosphohydrolase 1, ATP diphosphohydrolase, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, Nucleoside triphosphate diphosphohydrolase 1, ATP-DPH, ATPDase, Ecto-ATPDase 1, Ecto-ATPase 1, NTPDase1
Recommended products
Rabbit Recombinant Monoclonal CD39 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20461
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab232537 is the carrier-free version of Anti-CD39 antibody [EPR20461] ab223843.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65540.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD39 with Anti-CD39 antibody [EPR20461] ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human pancreas (PMID: 18567823; PMID: 19308674). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR20461] ab223843).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49730.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with Anti-CD39 antibody [EPR20461] ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR20461] ab223843).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img140404.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD39 with Anti-CD39 antibody [EPR20461] ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human tonsil (PMID: 27532024). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR20461] ab223843).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr20461-bsa-and-azide-free-ab232537--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img427937.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)")
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (ab232537)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed human tonsil tissue staining with ab232537 at 5µg/ml dilution. Secondary antibody was Alexa Fluor® 555 Anti-Rabbit IgG at 2 µg/ml. Samples were incubated with the primary antibody with TBS + 0.1% Tween, 3% BSA, 5% horse serum for 16 hours at 4°C. Blocking was done using 3% BSA, 5% horse serum for 1 hour at 20 degrees. Heat mediated antigen retrieval with 10mM Tris Base, 1mM EDTA, pH 9.2.
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