Anti-CD39 antibody [EPR20461] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID : 25403716). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223843).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human pancreas (PMID : 18567823; PMID : 19308674). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223843).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD39 with ab223843 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human tonsil (PMID : 27532024). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223843).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

AbReview82605****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20461] - BSA and Azide free (AB232537)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed human tonsil tissue staining with ab232537 at 5µg/ml dilution. Secondary antibody was Alexa Fluor® 555 Anti-Rabbit IgG at 2 µg/ml. Samples were incubated with the primary antibody with TBS + 0.1% Tween, 3% BSA, 5% horse serum for 16 hours at 4°C. Blocking was done using 3% BSA, 5% horse serum for 1 hour at 20 degrees. Heat mediated antigen retrieval with 10mM Tris Base, 1mM EDTA, pH 9.2.

This image is courtesy of an anonymous Abreview