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AB223842

Anti-CD39 antibody [EPR20627]

5

(6 Reviews)

|

(24 Publications)

Anti-CD39 antibody [EPR20627] (ab223842) is a rabbit monoclonal antibody detecting CD39 in Western Blot, IHC-P. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD39, ENTPD1, Ectonucleoside triphosphate diphosphohydrolase 1, ATP diphosphohydrolase, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, Nucleoside triphosphate diphosphohydrolase 1, ATP-DPH, ATPDase, Ecto-ATPDase 1, Ecto-ATPase 1, NTPDase1

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)

Tissue Microarrays stained for "Anti-CD39 antibody [EPR20627]" using "ab223842"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab223842 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID : 25403716). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human spleen (PMID : 22684996; PMID : 27532024). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human liver (PMID : 18567823; PMID : 19308674). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse spleen (PMID : 22684996; PMID : 27532024). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842)

Tissue Microarrays stained for "Anti-CD39 antibody [EPR20627]" using "ab223842"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab223842 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Western blot - Anti-CD39 antibody [EPR20627] (AB223842)
  • WB

Supplier Data

Western blot - Anti-CD39 antibody [EPR20627] (AB223842)

Exposure time : Lane 1 : 30 seconds; Lane 2 : 3 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 18036594; PMID : 10636909).

Lanes 1 - 2:

Western blot - Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution

Lanes 3 - 5:

Western blot - Anti-CD39 antibody [EPR20627] (ab223842) at 1/10000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human placenta lysate at 20 µg

Lane 3:

Human fetal spleen lysate at 20 µg

Lane 4:

Human tonsil lysate at 20 µg

Lane 5:

Mouse placenta lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 78 kDa

true

Western blot - Anti-CD39 antibody [EPR20627] (AB223842)
  • WB

Supplier Data

Western blot - Anti-CD39 antibody [EPR20627] (AB223842)

Exposure times : Lane 1 : 30 seconds; Lane 2 : 3 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 18036594; PMID : 10636909).

All lanes:

Western blot - Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse heart lysate at 20 µg

Lane 3:

Mouse kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 58 kDa

Observed band size: 78 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20627

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-CD39 antibody [EPR20627] (ab223842) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of CD39?
Anti-CD39 [EPR20627] (ab223842) specifically detects a band for CD39 (UniProt: P49961) at a molecular weight of 57kDa.

Reviewed by scientists
Anti-CD39 [EPR20627] (ab223842) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20627] also available for your convenience: ab223842, Carrier free - ab236038, Carrier free - ab302868, Alexa Fluor® 647 - ab312897, Alexa Fluor® 488 - ab313728

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides (Probable) (PubMed : 8529670, PubMed : 8626624, PubMed : 8955160, PubMed : 8996251). By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow (PubMed : 8955160, PubMed : 8996251).
See full target information ENTPD1
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:9635 PubMed40113898

2025

mTOR Inhibition limits LPS induced acute kidney injury and ameliorates hallmarks of cellular senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Stasi,Rossana Franzin,Fabio Sallustio,Alessandro Scagliotti,Paola Cappello,Elena Squiccimarro,Gianvito Caggiano,Rosa Losapio,Monica Campioni,Antonino Castellaneta,Vincenzo Cantaluppi,Claudia Curci,Paola Pontrelli,Giovanni Stallone,Loreto Gesualdo,Vito Fanelli,Giuseppe Castellano

Cell reports. Medicine 6:101934 PubMed39909044

2025

Spatially resolved transcriptomics reveal the determinants of primary resistance to immunotherapy in NSCLC with mature tertiary lymphoid structures.

Applications

Unspecified application

Species

Unspecified reactive species

Florent Peyraud,Jean-Philippe Guégan,Christophe Rey,Oren Lara,Ophélie Odin,Marie Del Castillo,Lucile Vanhersecke,Jean-Michel Coindre,Emma Clot,Maxime Brunet,Thomas Grellety,Angélique Tasseel,Sylvestre Le Moulec,Robert J Johnston,Alban Bessede,Antoine Italiano

iScience 27:111488 PubMed39759009

2025

Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junyang Li,Bingjie Mei,Lu Feng,Xiaoxin Wang,Dengfeng Wang,Jianming Huang,Guonan Zhang

Journal of thoracic disease 16:6094-6100 PubMed39444859

2024

Characteristics and outcomes of salvage surgery after immune checkpoint inhibitor therapy for initially unresectable non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masatsugu Hamaji,Hiroaki Ozasa,Yuichi Sakamori,Kazuhiro Terada,Akihiko Yoshizawa,Ryutaro Kikuchi,Yasuto Sakaguchi,Makoto Sonobe,Yusuke Muranishi,Ryo Miyahara,Hideki Motoyama,Mitsugu Omasa,Hiroshi Date

Nature communications 15:9033 PubMed39426955

2024

Spatial dynamics of CD39CD8 exhausted T cell reveal tertiary lymphoid structures-mediated response to PD-1 blockade in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenro Tanoue,Hirofumi Ohmura,Koki Uehara,Mamoru Ito,Kyoko Yamaguchi,Kenji Tsuchihashi,Yudai Shinohara,Peng Lu,Shingo Tamura,Hozumi Shimokawa,Taichi Isobe,Hiroshi Ariyama,Yoshihiro Shibata,Risa Tanaka,Hitoshi Kusaba,Taito Esaki,Kenji Mitsugi,Daisuke Kiyozawa,Takeshi Iwasaki,Hidetaka Yamamoto,Yoshinao Oda,Koichi Akashi,Eishi Baba

Journal of nanobiotechnology 22:563 PubMed39272146

2024

Circ_0001947 encapsulated by small extracellular vesicles promotes gastric cancer progression and anti-PD-1 resistance by modulating CD8 T cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyu Wang,Wenbo Liu,Mingming Zhang,Yong Li,Hongyue Tang,Yingying Wang,Chao Song,Buyun Song,Bibo Tan

Oncology letters 28:368 PubMed38933811

2024

Synergistic upregulation of PD‑L1 in tumor cells and CD39 in tumor‑infiltrating CD8 T cells leads to poor prognosis in patients with hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Kang,Sinan Zhao,Shan Lin,Jing Li,Shunxiang Wang

Molecular cancer therapeutics 23:1471-1482 PubMed38797955

2024

Characterization of AB598, a CD39 Enzymatic Inhibitory Antibody for the Treatment of Solid Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Amy E Anderson,Kaustubh Parashar,Ke Jin,Julie Clor,Carlo E Stagnaro,Urvi Vani,Jaskirat Singh,Ada Chen,Yihong Guan,Priyanka Talukdar,Pavithra Sathishkumar,Damie J Juat,Hema Singh,Ritu Kushwaha,Xiaoning Zhao,Angelo Kaplan,Lisa Seitz,Matthew J Walters,Ester Fernandez-Salas,Nigel P C Walker,Christine E Bowman

Scientific reports 14:11609 PubMed38773214

2024

Uracil as a biomarker for spatial pyrimidine metabolism in the development of gingivobuccal oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Soni Shaikh,Sangramjit Basu,Swarnendu Bag,Ankita Chatterjee,Sourav Datta,Devmalya Banerjee,Kapila Manikantan,Indu Arun,Pattatheyil Arun,Nidhan K Biswas,Arindam Maitra,Deepak Kumar Mishra,Partha P Majumder,Harsh Dhar,Geetashree Mukherjee

Cell reports. Medicine 5:101505 PubMed38614095

2024

Macrophage-coated tumor cluster aggravates hepatoma invasion and immunotherapy resistance via generating local immune deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Junya Ning,Yingnan Ye,Hongru Shen,Runjiao Zhang,Huikai Li,Tianqiang Song,Rui Zhang,Pengpeng Liu,Guidong Chen,Hailong Wang,Fenglin Zang,Xiangchun Li,Jinpu Yu
View all publications
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Product promise

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