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Rabbit Recombinant Monoclonal CD39 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications.


Images

Western blot - Anti-CD39 antibody [EPR20627] (AB223842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (AB223842), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

Alternative names

Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, Ecto-ATPDase 1, Ecto-ATPase 1, ENTPD1, CD39

Recommended products

Rabbit Recombinant Monoclonal CD39 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications.

Alternative names

Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, Ecto-ATPDase 1, Ecto-ATPase 1, ENTPD1, CD39

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20627

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Western blot - Anti-CD39 antibody [EPR20627] (ab223842)

    Exposure time : Lane 1: 30 seconds; Lane 2: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18036594; PMID: 10636909).

    Lanes 1 - 2: Western blot - Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution

    Lanes 3 - 5: Western blot - Anti-CD39 antibody [EPR20627] (ab223842) at 1/10000 dilution

    Lane 1: Human fetal heart lysate at 20 µg

    Lane 2: Human placenta lysate at 20 µg

    Lane 3: Human fetal spleen lysate at 20 µg

    Lane 4: Human tonsil lysate at 20 µg

    Lane 5: Mouse placenta lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa

    Observed band size: 78 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human liver (PMID: 18567823; PMID: 19308674). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Western blot - Anti-CD39 antibody [EPR20627] (ab223842)

    Exposure times: Lane 1: 30 seconds; Lane 2: 3 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18036594; PMID: 10636909).

    All lanes: Western blot - Anti-CD39 antibody [EPR20627] (ab223842) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 20 µg

    Lane 2: Mouse heart lysate at 20 µg

    Lane 3: Mouse kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 58 kDa

    Observed band size: 78 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

    Tissue Microarrays stained for "Anti-CD39 antibody [EPR20627]" using "ab223842"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab223842 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] (ab223842)

    Tissue Microarrays stained for "Anti-CD39 antibody [EPR20627]" using "ab223842"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with ab223842 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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