Anti-CD39 antibody [EPR20627] - BSA and Azide free

7 product images

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  This image is courtesy of the Single Cell & Imaging Mass Cytometry Analysis Platform, Goodman Cancer Research Centre, McGill University

  Mass Cytometry - Anti-CD39 antibody [EPR20627] - BSA and Azide free (ab236038)

  Imaging Mass Cytometry™ (IMC™) image of human tonsil tissue stained with Anti-CD39 antibody [EPR20627]. ab236038 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm's protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.
  

  Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] - BSA and Azide free (ab236038)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD39 with Anti-CD39 antibody [EPR20627] ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR20627] ab223842).

  Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] - BSA and Azide free (ab236038)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with Anti-CD39 antibody [EPR20627] ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse spleen (PMID: 22684996; PMID: 27532024). Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR20627] ab223842).

  Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] - BSA and Azide free (ab236038)

  Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD39 with Anti-CD39 antibody [EPR20627] ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on endothelial cells of human liver (PMID: 18567823; PMID: 19308674). Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR20627] ab223842).

  Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] - BSA and Azide free (ab236038)

  Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CD39 with Anti-CD39 antibody [EPR20627] ab223842 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on stromal cells of human colon carcinoma (PMID: 25403716). Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR20627] ab223842).

  Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] - BSA and Azide free (ab236038)

  Tissue Microarrays stained for "Anti-CD39 antibody [EPR20627]" using "Anti-CD39 antibody [EPR20627] ab223842"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with Anti-CD39 antibody [EPR20627] ab223842 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR20627] - BSA and Azide free (ab236038)

  Tissue Microarrays stained for "Anti-CD39 antibody [EPR20627]" using "Anti-CD39 antibody [EPR20627] ab223842"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). The sections were incubated with Anti-CD39 antibody [EPR20627] ab223842 at +4°C overnight followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).