Rabbit Recombinant Monoclonal CD39 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-CD39 antibody [EPR22507-67] - BSA and Azide free (AB254008), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr22507-67-bsa-and-azide-free-ab254008--western-blot-img38403.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD39 antibody [EPR22507-67] - BSA and Azide free (AB254008)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides. By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow.
Alternative names
CD39, Cd39, Entpd1, Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, ATP diphosphohydrolase, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, Nucleoside triphosphate diphosphohydrolase 1, ATP-DPH, ATPDase, Ecto-ATPDase 1, Ecto-ATPase 1, NTPDase1
Recommended products
Rabbit Recombinant Monoclonal CD39 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22507-67
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab254008 is the carrier-free version of Anti-CD39 antibody [EPR22507-67] ab252917.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-CD39 antibody [EPR22507-67] - BSA and Azide free (ab254008), expandable thumbnail](https://content.abcam.com/products/images/cd39-antibody-epr22507-67-bsa-and-azide-free-ab254008--western-blot-img38403.jpg "Western blot - Anti-CD39 antibody [EPR22507-67] - BSA and Azide free (ab254008)")
Western blot - Anti-CD39 antibody [EPR22507-67] - BSA and Azide free (ab254008)
The molecular weight observed is consistent with what has been described in the literature (PMID:18036594).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 37 secs; Lane 2: 48 secs; Lanes 3-4: 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD39 antibody [EPR22507-67] ab252917).
All lanes: Western blot - Anti-CD39 antibody [EPR22507-67] (Anti-CD39 antibody [EPR22507-67] ab252917) at 1/1000 dilution
Lane 1: Mouse heart lysate at 20 µg
Lane 2: Mouse placenta lysate at 20 µg
Lane 3: Rat spleen lysate at 20 µg
Lane 4: Rat heart lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 78 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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