AB103573

Anti-CD3D antibody

Name: Anti-CD3D antibody (ab103573) | Abcam
Brand: Abcam Limited
SKU: AB103573
Price: 545 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CD3d antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD3D.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD3D antibody (AB103573)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD3D antibody (AB103573)

Proximity Ligation Analysis (PLA) of protein-protein interactions between CD3D and CANX.
ab103573 at 1/1200 staining CDED in HeLa cells with anti-CANX mouse monoclonal antibody 1/50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

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Western blot - Anti-CD3D antibody (AB103573)

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Western blot - Anti-CD3D antibody (ab103573) at 1/500 dilution

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CD20 transfected 293T cell line lysate at 25 µg

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Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 19 kDa

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Western blot - Anti-CD3D antibody (AB103573)

All lanes:

Western blot - Anti-CD3D antibody (ab103573) at 1/500 dilution

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Mouse Kidney tissue lysate at 50 µg

Predicted band size: 19 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CD3D.

P04234

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).

See full target information CD3D

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed34491908

2021

CD146 bound to LCK promotes T cell receptor signaling and antitumor immune responses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxia Duan,Lin Jing,Xiaoqing Jiang,Yanbin Ma,Daji Wang,Jianquan Xiang,Xuehui Chen,Zhenzhen Wu,Huiwen Yan,Junying Jia,Zheng Liu,Jing Feng,Mingzhao Zhu,Xiyun Yan

bioRxiv : the preprint server for biology : PubMed34013264

2020

Infection of human lymphomononuclear cells by SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Marjorie C Pontelli,Italo A Castro,Ronaldo B Martins,Flávio P Veras,Leonardo La Serra,Daniele C Nascimento,Ricardo S Cardoso,Roberta Rosales,Thais M Lima,Juliano P Souza,Diego B Caetité,Mikhael H F de Lima,Juliana T Kawahisa,Marcela C Giannini,Letícia P Bonjorno,Maria I F Lopes,Sabrina S Batah,Li Siyuan,Rodrigo L Assad,Sergio C L Almeida,Fabiola R Oliveira,Maíra N Benatti,Lorena L F Pontes,Rodrigo C Santana,Fernando C Vilar,Maria A Martins,Thiago M Cunha,Rodrigo T Calado,José C Alves-Filho,Dario S Zamboni,Alexandre Fabro,Paulo Louzada-Junior,Rene D R Oliveira,Fernando Q Cunha,Eurico Arruda

European journal of immunology 49:1278-1290 PubMed31054264

2019

Engineering antigen-specific NK cell lines against the melanoma-associated antigen tyrosinase via TCR gene transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Ayhan Parlar,Ece Canan Sayitoglu,Didem Ozkazanc,Anna-Maria Georgoudaki,Cevriye Pamukcu,Mertkaya Aras,Benjamin J Josey,Michael Chrobok,Suzanne Branecki,Pegah Zahedimaram,Lolai Ikromzoda,Evren Alici,Batu Erman,Adil D Duru,Tolga Sutlu

Molecular cell 67:322-333.e6 PubMed28689658

2017

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Lu,Jiayi Wu,Yuanchen Dong,Shuobing Chen,Shuangwu Sun,Yong-Bei Ma,Qi Ouyang,Daniel Finley,Marc W Kirschner,Youdong Mao

View all publications

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