Rabbit Recombinant Monoclonal CD3d antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, Flow Cyt, WB and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--flow-cytometry-img165103.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040)")
![Western blot - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--western-blot-img172470.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040)")
![Immunoprecipitation - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--immunoprecipitation-img4083.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--immunocytochemistry-immunofluorescence-img165951.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165563.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (AB215040)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Version
3 products for Alternative Product
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
Alternative names
CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
Recommended products
Rabbit Recombinant Monoclonal CD3d antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, Flow Cyt, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP4426
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab215040 is the carrier-free version of Anti-CD3D antibody [EP4426] ab109531.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--flow-cytometry-img165103.jpg "Flow Cytometry - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)")
Flow Cytometry - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)
Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD3D with Purified Anti-CD3D antibody [EP4426] ab109531 at 1:20 dilution (5μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using Anti-CD3D antibody [EP4426] ab109531, the same antibody clone in a different buffer formulation.
![Western blot - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--western-blot-img172470.jpg "Western blot - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)")
Western blot - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)
This data was developed using Anti-CD3D antibody [EP4426] ab109531, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CD3D antibody [EP4426] (Anti-CD3D antibody [EP4426] ab109531) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2: HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 19 kDa
![Immunoprecipitation - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--immunoprecipitation-img4083.jpg "Immunoprecipitation - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)")
Immunoprecipitation - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)
Purified Anti-CD3D antibody [EP4426] ab109531 at 1/50 dilution (2μg) immunoprecipitating CD3D in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+): Anti-CD3D antibody [EP4426] ab109531 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD3D antibody [EP4426] ab109531 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDaThis data was developed using Anti-CD3D antibody [EP4426] ab109531, the same antibody clone in a different buffer formulation.
All lanes: Immunoprecipitation - Anti-CD3D antibody [EP4426] (Anti-CD3D antibody [EP4426] ab109531)
Predicted band size: 19 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--immunocytochemistry-immunofluorescence-img165951.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)")
Immunocytochemistry/ Immunofluorescence - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)
Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD3D with Purified Anti-CD3D antibody [EP4426] ab109531 at 1:50 dilution (2.6 μg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using Anti-CD3D antibody [EP4426] ab109531, the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040), expandable thumbnail](https://content.abcam.com/products/images/cd3d-antibody-ep4426-low-endotoxin-azide-free-ab215040--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165563.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free (ab215040)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD3D with Purified Anti-CD3D antibody [EP4426] ab109531 at 1:100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. M3
This data was developed using Anti-CD3D antibody [EP4426] ab109531, the same antibody clone in a different buffer formulation.
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