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Rabbit Recombinant Monoclonal CD3d antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-CD3D antibody [EPR20544] (AB213362), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] (AB213362), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD3D antibody [EPR20544] (AB213362), expandable thumbnail
  • Western blot - Anti-CD3D antibody [EPR20544] (AB213362), expandable thumbnail
  • Immunoprecipitation - Anti-CD3D antibody [EPR20544] (AB213362), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-FrIHC-P
Mouse
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/600
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed:10935641). Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.

Alternative names

CD3d, T3d, Cd3d, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

Recommended products

Rabbit Recombinant Monoclonal CD3d antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20544
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-CD3D antibody [EPR20544] (ab213362), expandable thumbnail

    Flow Cytometry - Anti-CD3D antibody [EPR20544] (ab213362)

    Flow cytometric analysis of mouse spleen cells labeling CD3D with ab213362 at 1/600 dilution (right panel), compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Firstly, cells surface was stained with anti-mouse CD3 Alexa Fluor 647 (Y axis), then cells were fixed with 4% PFA followed by intracellular staining with ab213362. The same population of cells were stained by both: anti-mouse CD3 and anti-mouse CD3D (ab213362) antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] (ab213362), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] (ab213362)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3D with ab213362 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Cytoplasmic staining on T cells of mouse splenic periarterial lymphatic sheath is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-CD3D antibody [EPR20544] (ab213362), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD3D antibody [EPR20544] (ab213362)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD3D with ab213362 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic and membranous staining on mouse splenic periarterial lymphatic sheath is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Western blot - Anti-CD3D antibody [EPR20544] (ab213362), expandable thumbnail

    Western blot - Anti-CD3D antibody [EPR20544] (ab213362)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 15 seconds; Lane 2: 3 minutes.

    All lanes: Western blot - Anti-CD3D antibody [EPR20544] (ab213362) at 1/1000 dilution

    Lane 1: Mouse thymus tissue lysate at 20 µg

    Lane 2: Mouse lymph node tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 19 kDa

    Observed band size: 24 kDa

  • Immunoprecipitation - Anti-CD3D antibody [EPR20544] (ab213362), expandable thumbnail

    Immunoprecipitation - Anti-CD3D antibody [EPR20544] (ab213362)

    CD3D was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab213362 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213362 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse thymus lysate, 10 μg (Input).

    Lane 2: ab213362 IP in mouse thymus lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab213362 in mouse thymus lysate .

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-CD3D antibody [EPR20544] (ab213362)

    Predicted band size: 19 kDa

    Observed band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] (ab213362), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3D antibody [EPR20544] (ab213362)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD3D with ab213362 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Cytoplasmic and membranous staining on T cells of mouse thymus medulla is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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