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AB108996

Anti-CD3G antibody [EPR4516]

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(2 Publications)

Rabbit Recombinant Monoclonal CD3G antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD3g, T3G, CD3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain

3 Images
Western blot - Anti-CD3G antibody [EPR4516] (AB108996)
  • WB

Unknown

Western blot - Anti-CD3G antibody [EPR4516] (AB108996)

All lanes:

Western blot - Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human fetal thymus tissue lysate at 10 µg

Lane 3:

HuT-78 cell lysate at 10 µg

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Western blot - Anti-CD3G antibody [EPR4516] (AB108996)
  • WB

Unknown

Western blot - Anti-CD3G antibody [EPR4516] (AB108996)

All lanes:

Western blot - Anti-CD3G antibody [EPR4516] (ab108996) at 1/1000 dilution

All lanes:

Jurkat cell lysate at 10 µg

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3G antibody [EPR4516] (AB108996)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3G antibody [EPR4516] (AB108996)

ab108996 at 1/100 dilution staining CD3G in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Carrier free

    Anti-CD3G antibody [EPR4516] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4516

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed : 8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
See full target information CD3G
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 26:126 PubMed33297933

2020

The involvement of neuroimmune cells in adipose innervation.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Blaszkiewicz,Elizabeth Wood,Sigi Koizar,Jake Willows,Ryan Anderson,Yu-Hua Tseng,James Godwin,Kristy L Townsend

EBioMedicine 24:216-230 PubMed28888925

2017

Follicular Helper T Cells are Essential for the Elimination of Plasmodium Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Damián Pérez-Mazliah,Minh Phuong Nguyen,Caroline Hosking,Sarah McLaughlin,Matthew D Lewis,Irene Tumwine,Prisca Levy,Jean Langhorne
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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