JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247738

Anti-CD3G antibody [EPR4516] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CD3G antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CD3g, T3G, CD3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3G antibody [EPR4516] - BSA and Azide free (AB247738)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3G antibody [EPR4516] - BSA and Azide free (AB247738)

This data was developed using ab108996, the same antibody clone in a different buffer formulation.

ab108996, at 1/100 dilution, staining CD3G in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CD3G antibody [EPR4516] - BSA and Azide free (AB247738)
  • WB

Unknown

Western blot - Anti-CD3G antibody [EPR4516] - BSA and Azide free (AB247738)

This data was developed using ab108996, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD3G antibody [EPR4516] (<a href='/en-us/products/primary-antibodies/cd3g-antibody-epr4516-ab108996'>ab108996</a>) at 1/1000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Predicted band size: 20 kDa

false

Western blot - Anti-CD3G antibody [EPR4516] - BSA and Azide free (AB247738)
  • WB

Unknown

Western blot - Anti-CD3G antibody [EPR4516] - BSA and Azide free (AB247738)

This data was developed using ab108996, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD3G antibody [EPR4516] (<a href='/en-us/products/primary-antibodies/cd3g-antibody-epr4516-ab108996'>ab108996</a>) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human fetal thymus tissue lysate at 10 µg

Lane 3:

HuT-78 cell lysate at 10 µg

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4516

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab247738 is the carrier-free version of ab108996.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed : 8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
See full target information CD3G
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 79
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 19
Primary Antibodies

AB32070

Anti-LAT antibody [Y109]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-LAT antibody [Y109] (AB32070)

  • 0
  • 0
Primary Antibodies

AB237709

Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] (AB237709)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com