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Anti-CD4 antibody [CAL4] (ab237722) is a rabbit monoclonal antibody that is used to detect CD4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr. Suitable for Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (AB237722), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (AB237722), expandable thumbnail
  • Western blot - Anti-CD4 antibody [CAL4] (AB237722), expandable thumbnail
  • Immunoprecipitation - Anti-CD4 antibody [CAL4] (AB237722), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [CAL4] (AB237722), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Mouse
Tested
Tested
Not recommended
Tested
Tested
Tested
Rat
Expected
Tested
Not recommended
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/40
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

Alternative names

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

Recommended products

Anti-CD4 antibody [CAL4] (ab237722) is a rabbit monoclonal antibody that is used to detect CD4 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr. Suitable for Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
CAL4
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

Purity >99%.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-CD4 antibody [CAL4] (ab237722) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), in Mouse, Rat samples.

What is the molecular weight of CD4?


Anti-CD4 [CAL4] (ab237722) specifically detects a band for CD4 (UniProt: P06332) at a molecular weight of 51kDa.

Recommended positive controls


IHC-P: Mouse and rat spleen tissue.WB: Mouse and rat thymus, spleen and lymph node lysate.IHC-Fr: Mouse spleen tissue.Flow Cyt (intra): Mouse splenocytes.IP: Mouse thymus lysate.

Trusted by the scientific community


Anti-CD4 [CAL4] (ab237722) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Other related products


We have a range of other formats of antibody clone [CAL4] also available for your convenience:
ab237722, Carrier free - Anti-CD4 antibody [CAL4] - BSA and Azide free ab251608



Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD4 with ab237722 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse spleen. The section was incubated with ab237722 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD4 with ab237722 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat spleen. The section was incubated with ab237722 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Western blot - Anti-CD4 antibody [CAL4] (ab237722), expandable thumbnail

    Western blot - Anti-CD4 antibody [CAL4] (ab237722)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD4 antibody [CAL4] (ab237722) at 1/1000 dilution

    Lane 1: Mouse thymus lysate at 20 µg

    Lane 2: Mouse spleen lysate at 20 µg

    Lane 3: Mouse lymph node lysate at 20 µg

    Lane 4: Rat thymus lysate at 20 µg

    Lane 5: Rat spleen lysate at 20 µg

    Lane 6: Rat lymph node lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa

  • Immunoprecipitation - Anti-CD4 antibody [CAL4] (ab237722), expandable thumbnail

    Immunoprecipitation - Anti-CD4 antibody [CAL4] (ab237722)

    CD4 was immunoprecipitated from 0.35 mg mouse thymus lysate with ab237722 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237722 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used at 1/5000 dilution.
    Lane 1: Mouse thymus lysate 10 μg (Input).
    Lane 2: ab237722 IP in mouse thymus lysate .
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab237722 in mouse thymus lysate .
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    102kDa band represents CD4 dimer based on literature. (PMID: 20739067).

    All lanes: Immunoprecipitation - Anti-CD4 antibody [CAL4] (ab237722)

    Predicted band size: 51 kDa

  • Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [CAL4] (ab237722), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [CAL4] (ab237722)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD4 with ab237722 at 1/100 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on T cell surface (PMID: 26994219) is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Flow Cytometry (Intracellular) - Anti-CD4 antibody [CAL4] (ab237722), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD4 antibody [CAL4] (ab237722)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes labeling CD4 with ab237722 at 1/40 (right panel) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min and intracellularly stained with rabbit IgG (Left) or ab237722 (Right). They recognize the same cell population.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] (ab237722)

    Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab237722 at 0.5 μg/ml in immunohistochemical analysis.

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