Rabbit Recombinant Monoclonal CD4 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 10 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-frozen-sections-img53680.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)")
![Immunoprecipitation - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunoprecipitation-img54600.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87209.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95266.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136099.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (AB221775)")
Publications
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- ACS bio & med chem Au 3:97-1132023TLR4 Blockade Using Docosahexaenoic Acid Restores Vulnerability of Drug-Tolerant Tumor Cells and Prevents Breast Cancer Metastasis and Postsurgical Relapse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMou Wang,Yuejing Wang,Renhe Liu,Ruilian Yu,Tao Gong,Zhirong Zhang,Yao FuPubMed 37101603
- Molecular oncology 16:485-5072021Recombinant nanobody against MUC1 tandem repeats inhibits growth, invasion, metastasis, and vascularization of spontaneous mouse mammary tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParnaz Merikhian,Behrad Darvishi,Neda Jalili,Mohammad Reza Esmailinejad,Azadeh Sharif Khatibi,Shima Moradi Kalbolandi,Malihe Salehi,Marjan Mosayebzadeh,Mahdieh Shokrollahi Barough,Keivan Majidzadeh-A,Fatemeh Yadegari,Fatemeh Rahbarizadeh,Leila FarahmandPubMed 34694686
- Journal of biological engineering 15:202021Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMitra Samadi,Keivan Majidzadeh-A,Malihe Salehi,Neda Jalili,Zeinab Noorinejad,Marjan Mosayebzadeh,Ahad Muhammadnejad,Azadeh Sharif Khatibi,Shima Moradi-Kalbolandi,Leila FarahmandPubMed 34344421
- Scientific reports 11:95632021Cholinergic stimulation with pyridostigmine modulates a heart-spleen axis after acute myocardial infarction in spontaneous hypertensive rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobson Luiz Bandoni,Pamela Nithzi Bricher Choque,Humberto Dellê,Tercio Lemos de Moraes,Maria Helena Mattos Porter,Bruno Durante da Silva,Gizele Alves Neves,Maria-Claudia Irigoyen,Kátia De Angelis,Valentin A Pavlov,Luis Ulloa,Fernanda Marciano Consolim-ColomboPubMed 33953291
- EBioMedicine 67:1033452021Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyuhei Okada,Aki Furusawa,Daniel W Vermeer,Fuyuki Inagaki,Hiroaki Wakiyama,Takuya Kato,Tadanobu Nagaya,Peter L Choyke,William C Spanos,Clint T Allen,Hisataka KobayashiPubMed 33933782
- Breast cancer research : BCR 20:662018Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColleen Chute et. al.PubMed 29976229
- Cell death & disease 8:e26952017The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury.
- Molecular genetics and metabolism reports 9:54-602016Liver-specific knockout of arginase-1 leads to a profound phenotype similar to inducible whole body arginase-1 deficiency.
- Cancer immunology, immunotherapy : CII 65:1401-14102016Regulatory T cells function at the early stage of tumor progression in a mouse model of tongue squamous cell carcinoma.
- Journal of clinical & cellular immunology 7:2016Development of inCVAX, Cancer Vaccine, and Its Immune Response in Mice with Hepatocellular Cancer.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Mouse | Tested | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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15 products for Alternative Product
4 products for Alternative Version
Target data
Function
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
Alternative names
CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4
Recommended products
Rabbit Recombinant Monoclonal CD4 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19514
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab221775 is the carrier-free version of Anti-CD4 antibody [EPR19514] ab183685.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-frozen-sections-img53680.jpg "Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
This IHC data was generated using the same anti-CD4 antibody clone, EPR19514, in a different buffer formulation (cat# Anti-CD4 antibody [EPR19514] ab183685).
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CD4 with Anti-CD4 antibody [EPR19514] ab183685 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
The result showed membrane staining on mouse spleen.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
![Immunoprecipitation - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunoprecipitation-img54600.jpg "Immunoprecipitation - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
Immunoprecipitation - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
CD4 was immunoprecipitated from 1mg of Mouse thymus whole cell lysate with Anti-CD4 antibody [EPR19514] ab183685 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using Anti-CD4 antibody [EPR19514] ab183685 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse thymus whole cell lysate, 10μg (Input).
Lane 2: Anti-CD4 antibody [EPR19514] ab183685 IP in Mouse thymus whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD4 antibody [EPR19514] ab183685 in Mouse thymus whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD4 antibody [EPR19514] ab183685).
All lanes: Immunoprecipitation - Anti-CD4 antibody [EPR19514] (Anti-CD4 antibody [EPR19514] ab183685)
Predicted band size: 51 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img87209.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
Formaldehyde-fixed, paraffin-embedded mouse thymus tissue stained for CD4 using Anti-CD4 antibody [EPR19514] ab183685 at 1/500 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD4 antibody [EPR19514] ab183685).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95266.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
This IHC data was generated using the same anti-CD4 antibody clone, EPR19514, in a different buffer formulation (cat# Anti-CD4 antibody [EPR19514] ab183685).
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD4 with Anti-CD4 antibody [EPR19514] ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.Membrane staining on T cells is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136099.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CD4 with Anti-CD4 antibody [EPR19514] ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.Membrane staining on lymphocytes and negative on epithelium cells of mouse colon is observed.Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD4 antibody [EPR19514] ab183685).Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104648.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
10% NBF-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using Anti-CD4 antibody [EPR19514] ab183685 at 1/2000 dilution in immunohistochemical analysis, followed by Goat anti-Rabbit IgG Alexa Fluor® 647.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD4 antibody [EPR19514] ab183685).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59970.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CD4 with Anti-CD4 antibody [EPR19514] ab183685 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative on mouse cerebrum.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD4 antibody [EPR19514] ab183685).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104648.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775), expandable thumbnail](https://content.abcam.com/products/images/cd4-antibody-epr19514-low-endotoxin-azide-free-ab221775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59970.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)")
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