AB250678

Anti-CD4 antibody [EPR19533] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CD4 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, Cd4

2 Images

Supplier Data

Western blot - Anti-CD4 antibody [EPR19533] - BSA and Azide free (AB250678)

This data was developed using ab183686, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-8 : 3 minutes; Lane 9 : 5 seconds.

No band observed in lane 2 to lane 8 in our testing condition but CD4 should be specifically expressed in a few T cells.

All lanes:

Western blot - Anti-CD4 antibody [EPR19533] (<a href='/en-us/products/primary-antibodies/cd4-antibody-epr19533-ab183686'>ab183686</a>) at 1/1000 dilution

Lane 1:

Mouse thymus lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Mouse kidney lysate at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Lane 5:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 6:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 8:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 9:

Rat thymus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

Supplier Data

Western blot - Anti-CD4 antibody [EPR19533] - BSA and Azide free (AB250678)

This data was developed using ab183686, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CD4 antibody [EPR19533] (<a href='/en-us/products/primary-antibodies/cd4-antibody-epr19533-ab183686'>ab183686</a>) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa,51 kDa,52 kDa,66 kDa

Observed band size: 51 kDa,52 kDa,68 kDa

false

Exposure time: 3min

Unconjugated

Anti-CD4 antibody [EPR19533]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19533

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab250678 is the carrier-free version of ab183686.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

See full target information Cd4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 21:419 PubMed33841580

2021

Upregulation of circRNA_100395 sponges miR-142-3p to inhibit gastric cancer progression by targeting the PI3K/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyi Cheng,Guiyuan Liu,Chuanjiang Huang,Xiaojun Zhao

JCI insight 5: PubMed31094704

2019

Protective role of B cells in sterile particulate-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shaikh M Atif,Douglas G Mack,Amy S McKee,Javier Rangel-Moreno,Allison K Martin,Andrew Getahun,Lisa A Maier,John C Cambier,Rubin Tuder,Andrew P Fontenot

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD4 antibody [EPR19533] - BSA and Azide free

Western blot - Anti-CD4 antibody [EPR19533] - BSA and Azide free (AB250678)

Western blot - Anti-CD4 antibody [EPR19533] - BSA and Azide free (AB250678)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

Product promise