AB133313

Anti-CD4 antibody [EPR3942]

RabMAb
Recombinant
Lab Essentials
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal CD4 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images

Unknown

Western blot - Anti-CD4 antibody [EPR3942] (AB133313)

All lanes:

Western blot - Anti-CD4 antibody [EPR3942] (ab133313) at 1/1000 dilution

Lane 1:

Human tonsil lysates at 10 µg

Lane 2:

Fetal thymus lysates at 10 µg

Lane 3:

HuT-78 lysates at 10 µg

Lane 4:

Peripheral Blood Mononuclear Cell (PBMC) lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3942

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).

See full target information CD4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108162 PubMed39793889

2025

Axin-binding domain of glycogen synthase kinase 3β facilitates functional interactions with voltage-gated Na+ channel Na1.6.

Applications

Unspecified application

Species

Unspecified reactive species

Timothy John Baumgartner,Nolan Michael Dvorak,Nana Aboadwe Goode,Zahra Haghighijoo,Mate Marosi,Jully Singh,Aditya Kumar Singh,Fernanda Laezza

Nature communications 16:110 PubMed39747162

2025

Enhanced motivated behavior mediated by pharmacological targeting of the FGF14/Na1.6 complex in nucleus accumbens neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Nolan M Dvorak,Paul A Wadsworth,Guillermo Aquino-Miranda,Pingyuan Wang,Douglas S Engelke,Jingheng Zhou,Nghi Nguyen,Aditya K Singh,Giuseppe Aceto,Zahra Haghighijoo,Isabella I Smith,Nana Goode,Mingxiang Zhou,Yosef Avchalumov,Evan P Troendle,Cynthia M Tapia,Haiying Chen,Reid T Powell,Timothy J Baumgartner,Jully Singh,Leandra Koff,Jessica Di Re,Ann E Wadsworth,Mate Marosi,Marc R Azar,Kristina Elias,Paul Lehmann,Yorkiris M Mármol Contreras,Poonam Shah,Hector Gutierrez,Thomas A Green,Martin B Ulmschneider,Marcello D'Ascenzo,Clifford Stephan,Guohong Cui,Fabricio H Do Monte,Jia Zhou,Fernanda Laezza

The Journal of experimental medicine 221: PubMed38557723

2024

Helper T cell immunity in humans with inherited CD4 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Guérin,Marcela Moncada-Vélez,Katherine Jackson,Masato Ogishi,Jérémie Rosain,Mathieu Mancini,David Langlais,Andrea Nunez,Samantha Webster,Jesse Goyette,Taushif Khan,Nico Marr,Danielle T Avery,Geetha Rao,Tim Waterboer,Birgitta Michels,Esmeralda Neves,Cátia Iracema Morais,Jonathan London,Stéphanie Mestrallet,Pierre Quartier Dit Maire,Bénédicte Neven,Franck Rapaport,Yoann Seeleuthner,Atar Lev,Amos J Simon,Jorge Montoya,Ortal Barel,Julio Gómez-Rodríguez,Julio C Orrego,Anne-Sophie L'Honneur,Camille Soudée,Jessica Rojas,Alejandra C Velez,Irini Sereti,Benjamin Terrier,Nancy Marin,Luis F García,Laurent Abel,Stéphanie Boisson-Dupuis,Joel Reis,Antonio Marinho,Andrea Lisco,Emilia Faria,Christopher C Goodnow,Julia Vasconcelos,Vivien Béziat,Cindy S Ma,Raz Somech,Jean-Laurent Casanova,Jacinta Bustamante,Jose Luis Franco,Stuart G Tangye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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