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Mouse Monoclonal CD4 antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317797), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317797), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317797), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317797), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Target data

Function

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:12089508, PubMed:16331979, PubMed:2214026, PubMed:9641677). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).

Alternative names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

Recommended products

Mouse Monoclonal CD4 antibody. Carrier free. Suitable for IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6855
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317797 is the carrier-free version of Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) ab317787.

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-CD4 antibody [EPR6855] ab133616). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-CD4 antibody [EPR6855] ab133616).

    Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD4with Anti-CD4 antibody [EPR6855] ab133616 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with OptiView DAB IHC Detection Kit followed by OptiView Amplification kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). Anti-CD4 antibody [EPR6855] ab133616 anti-CD4 antibody [EPR6855] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-CD4 antibody [EPR6855] ab133616).

    Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD4with Anti-CD4 antibody [EPR6855] ab133616 at a concentration of 1.43 µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. Anti-CD4 antibody [EPR6855] ab133616 anti-CD4 antibody [EPR6855] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317797)

    Immunofluorescence staining of CD4 in sections of formalin-fixed paraffin-embedded human tonsil (positive) and human colon (negative)*.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317797 at 1/500 dilution, and then incubated for 1 hour with Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150112, Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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