AB33775

Anti-CD4 antibody [OX-35]

Lab Essentials
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(12 Publications)

Mouse Monoclonal CD4 antibody. Suitable for Flow Cyt and reacts with Rat samples. Cited in 12 publications. Immunogen corresponding to Cell preparation containing Cd4 protein.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen, Cd4

2 Images

Flow Cytometry - Anti-CD4 antibody [OX-35] (AB33775)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD4 antibody [OX-35] (AB33775)

Surface staining flow cytometric pattern of rat splenocytes stained with 1.6μg/ml ab33775 anti-rat CD4 purified antibody, GAM PE

Flow Cyt

Unknown

Flow Cytometry - Anti-CD4 antibody [OX-35] (AB33775)

Flow cytometric analysis using surface staining of rat splenocyte suspension. Rat splenocytes are stained using 1.6μg/ml ab33775 anti-rat CD4, GAM PE, (red) and are separated from REH cells stained with 1.6μg/ml mouse isotype control purified antibody

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX-35

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Cd4 protein. The exact immunogen used to generate this antibody is proprietary information.

P05540

Epitope

This antibody recognises a different epitope on the CD4 molecule than the W3/25 monoclonal (eg ab33779 and ab33783).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified by Protein A affinity chromatography

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

See full target information Cd4

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37175970

2023

Combined Supplementation of and Diminishes Chronic Unpredictable Mild Stress-Induced Intestinal Alterations via Activation of Nrf-2 Signaling Pathway in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sabiha Fatima,Haifa Altwaijry,Mahmoud M A Abulmeaty,Manal Abudawood,Nikhat J Siddiqi,Reem Hamoud Alrashoudi,Sarah Alsobaie

iScience 26:106647 PubMed37168574

2023

Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Dou Ye,Suqing Qu,Yinxiang Yang,Zhaoyan Wang,Qian Wang,Weipeng Liu,Fan Zhang,Qian Guan,Xiaohua Wang,Jing Zang,Xin Li,Hengtao Liu,Ruiqin Yao,Zhichun Feng,Zuo Luan

Journal of tissue engineering 13:20417314221118858 PubMed36003955

2022

Mesenchymal stem cells establish a pro-regenerative immune milieu after decellularized rat uterus tissue transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Edina Sehic,Emy Thorén,Ingigerdur Gudmundsdottir,Mihai Oltean,Mats Brännström,Mats Hellström

EJNMMI research 12:6 PubMed35107664

2022

Dimethyl fumarate decreases short-term but not long-term inflammation in a focal EAE model of neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

S K Vainio,A M Dickens,M Matilainen,F R López-Picón,R Aarnio,O Eskola,O Solin,D C Anthony,J O Rinne,L Airas,M Haaparanta-Solin

Movement disorders : official journal of the Movement Disorder Society 37:613-619 PubMed34766658

2021

Spontaneous Graft-Induced Dyskinesias Are Independent of 5-HT Neurons and Levodopa Priming in a Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Lane,David J Harrison,Elena Ramos-Varas,Rachel Hills,Sophie Turner,Mariah J Lelos

Journal of inflammation research 14:2979-2991 PubMed34262323

2021

Overexpression of Neutrophil MMP-9 and HIF-1α May Contribute to the Finger-Like Projections Formation and Histo-Pathogenesis in Nasal Inverted Papilloma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Kai Sen Tan,Yan Yi Tu,Li Zhao,Jing Liu,Hsiao Hui Ong,De Yun Wang,Li Shi

Experimental neurology 282:78-85 PubMed27235932

2016

hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation.

Applications

IHC

Species

Rat

Andreas Heuer,Agnete Kirkeby,Ulrich Pfisterer,Marie E Jönsson,Malin Parmar

American journal of physiology. Heart and circulat 305:H1761-71 PubMed24097428

2013

Inhibition of NF-κB activation by a novel IKK inhibitor reduces the severity of experimental autoimmune myocarditis via suppression of T-cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Watanabe,Ryoko Wakizono Azuma,Jun-ichi Suzuki,Masahito Ogawa,Akiko Itai,Yasunobu Hirata,Issei Komuro,Mitsuaki Isobe

Molecular therapy : the journal of the American So 21:1862-75 PubMed23799534

2013

Dose-dependent neuroprotection of VEGF₁₆₅ in Huntington's disease striatum.

Applications

Unspecified application

Species

Unspecified reactive species

Stuart M Ellison,Antonio Trabalza,Veronica Tisato,Evangelos Pazarentzos,Shirley Lee,Vasiliki Papadaki,Despoina Goniotaki,Sarah Morgan,Nazanin Mirzaei,Nicholas D Mazarakis

Artificial organs 37:600-5 PubMed23452255

2013

Pathological effects of processed bovine pericardial scaffolds--a comparative in vivo evaluation.

Applications

IHC

Species

Rat

Parvathy Thampi,Divakaran Nair,Lalithakunjamma R,Vijayan N,Syam Venugopal,Umashankar Ramachandra

View all publications

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