Anti-CD4 antibody [RM1136]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD4 antibody [RM1136] (AB316866)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling CD4 with ab316866 at 1/500 (1.066 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing membranous staining in subsets of human PBMCs (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-human CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt

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Flow Cytometry - Anti-CD4 antibody [RM1136] (AB316866)

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CD4 with ab316866 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

human PBMC are co-stained with CD3 conjugated Alexa Fluor®647.
Gated on viable cell.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [RM1136] (AB316866)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD4 with ab316866 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human tonsil. The section was incubated with ab316866 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [RM1136] (AB316866)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling CD4 with ab316866 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human Hodgkin lymphoma. The section was incubated with ab316866 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD4 antibody [RM1136] (AB316866)

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) cells labelling CD4 with ab316866 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

human PBMC are co-stained with CD8a conjugated Pacific Blue (450/50BP).
Gated on viable cell.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD4 antibody [RM1136] (AB316866)

Flow cytometric analysis of mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD4 with ab316866 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

mouse PBMC are co-stained with CD8a conjugated BV421 (440/50BP).
Gated on viable cell.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD4 antibody [RM1136] (AB316866)

Flow cytometric analysis of mouse PBMC (mouse peripheral blood mononuclear cell) cells labelling CD4 with ab316866 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

mouse PBMC are co-stained with CD3 conjugated Alexa Fluor®647.
Gated on viable cell.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [RM1136] (AB316866)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CD4 with ab316866 at 1/4000 (0.133 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : no staining on human skeletal muscle. The section was incubated with ab316866 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins