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AB244558

Anti-CD4 antibody [W3/25] - BSA and Azide free

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Mouse Monoclonal CD4 antibody. Carrier free. Suitable for Flow Cyt and reacts with Rat samples.

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images
Flow Cytometry - Anti-CD4 antibody [W3/25] - BSA and Azide free (AB244558)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD4 antibody [W3/25] - BSA and Azide free (AB244558)

This data was developed using the same antibody clone in a different buffer formulation (ab6413)

Lewis rat splenocytes stained with ab6413 (right) or mouse IgG1κ (ab170190) isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6413) or mouse IgG1κ (ab170190) (1x106 in 100 μl at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD3 antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

W3/25

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.2 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
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