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AB22469

Anti-CD40 antibody [3/23]

3

(1 Review)

|

(8 Publications)

Rat Monoclonal CD40 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cd40.

View Alternative Names

CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor

1 Images
Flow Cytometry - Anti-CD40 antibody [3/23] (AB22469)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD40 antibody [3/23] (AB22469)

ab22469 staining CD40 in Mouse Spleen cells by flow cytometric analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

3/23

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Mouse Cd40. The exact immunogen used to generate this antibody is proprietary information.

P27512

Specificity

Does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. Does not cross react with mouse T cells. ab22469 is a powerful activator of normal B cells especially in the presence of IL4.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg-isotype-control-low-endotoxin-azide-free-ab37371'>ab37371</a> - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed : 12881420).
See full target information Cd40
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:1027 PubMed36477438

2022

Lipid desaturation regulates the balance between self-renewal and differentiation in mouse blastocyst-derived stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chanchal Thomas Mannully,Reut Bruck-Haimson,Anish Zacharia,Paul Orih,Alaa Shehadeh,Daniel Saidemberg,Natalya M Kogan,Sivan Alfandary,Raphael Serruya,Arie Dagan,Isabelle Petit,Arieh Moussaieff

Journal of oncology 2022:9775736 PubMed36276271

2022

A Machine Learning Workflow of Multiplexed Immunofluorescence Images to Interrogate Activator and Tolerogenic Profiles of Conventional Type 1 Dendritic Cells Infiltrating Melanomas of Disease-Free and Metastatic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Saraí G De León Rodríguez,Paúl Hernández Herrera,Cristina Aguilar Flores,Vadim Pérez Koldenkova,Adán Guerrero,Alejandra Mantilla,Ezequiel M Fuentes-Pananá,Christopher Wood,Laura C Bonifaz

Annals of translational medicine 9:148 PubMed33569450

2021

Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Lu,Yong Liu,Xiaodan Wang,Haili Jiang,Zhaohui Liu

Experimental and therapeutic medicine 20:1541-1549 PubMed32742385

2020

Mechanism of TGF-β1 inhibiting Kupffer cell immune responses in cholestatic cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Qian,Yuwen Jiao,Guangyao Wang,Hanyang Liu,Xiang Cao,Haojun Yang

Cell stem cell 24:983-994.e7 PubMed31031139

2019

Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Benchetrit,Mohammad Jaber,Valery Zayat,Shulamit Sebban,Avital Pushett,Kirill Makedonski,Zvi Zakheim,Ahmed Radwan,Noam Maoz,Rachel Lasry,Noa Renous,Michal Inbar,Oren Ram,Tommy Kaplan,Yosef Buganim

eLife 6: PubMed29111976

2017

CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Carriba,Alun M Davies

Molecular therapy. Nucleic acids 4:e265 PubMed26623936

2015

CD40 Generation 2.5 Antisense Oligonucleotide Treatment Attenuates Doxorubicin-induced Nephropathy and Kidney Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron J Donner,Steve T Yeh,Gene Hung,Mark J Graham,Rosanne M Crooke,Adam E Mullick

Experimental neurology 229:372-80 PubMed21419121

2011

Effects of ethanol on transforming growth factor Β1-dependent and -independent mechanisms of neural stem cell apoptosis.

Applications

ICC, WB

Species

Mouse, Mouse

Steven D Hicks,Michael W Miller
View all publications
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