Rat Monoclonal CD40 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cd40.
Images
![Flow Cytometry - Anti-CD40 antibody [3/23] (AB22469), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-3-23-ab22469--flow-cytometry-img122161.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD40 antibody [3/23] (AB22469)")
Publications
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- Journal of oncology 2022:97757362022A Machine Learning Workflow of Multiplexed Immunofluorescence Images to Interrogate Activator and Tolerogenic Profiles of Conventional Type 1 Dendritic Cells Infiltrating Melanomas of Disease-Free and Metastatic Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaraí G De León Rodríguez,Paúl Hernández Herrera,Cristina Aguilar Flores,Vadim Pérez Koldenkova,Adán Guerrero,Alejandra Mantilla,Ezequiel M Fuentes-Pananá,Christopher Wood,Laura C BonifazPubMed 36276271
- Annals of translational medicine 9:1482021Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Lu,Yong Liu,Xiaodan Wang,Haili Jiang,Zhaohui LiuPubMed 33569450
- Experimental and therapeutic medicine 20:1541-15492020Mechanism of TGF-β1 inhibiting Kupffer cell immune responses in cholestatic cirrhosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Qian et. al.PubMed 32742385
- Cell stem cell 24:983-994.e72019Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Benchetrit et. al.PubMed 31031139
- eLife 6:2017CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaulina Carriba et. al.PubMed 29111976
- Molecular therapy. Nucleic acids 4:e2652015CD40 Generation 2.5 Antisense Oligonucleotide Treatment Attenuates Doxorubicin-induced Nephropathy and Kidney Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAaron J Donner et. al.PubMed 26623936
- Experimental neurology 229:372-802011Effects of ethanol on transforming growth factor Β1-dependent and -independent mechanisms of neural stem cell apoptosis.
Key facts
- Isotype
IgG
- Host species
Rat
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Mouse Cd40. The exact immunogen used to generate this antibody is proprietary information. Database link P27512
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µL for 106 Cells | Notes ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Alternative names
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, Cd40, Tnfrsf5
Recommended products
Rat Monoclonal CD40 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Cd40.
Key facts
- Isotype
IgG
- Host species
Rat
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Cd40. The exact immunogen used to generate this antibody is proprietary information. Database link P27512
- Clone number
3/23
- Purification technique
Affinity purification Protein G
- Specificity
Does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. Does not cross react with mouse T cells.
ab22469 is a powerful activator of normal B cells especially in the presence of IL4.- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-3-23-ab22469--flow-cytometry-img122161.jpg "Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)")
Flow Cytometry - Anti-CD40 antibody [3/23] (ab22469)
ab22469 staining CD40 in Mouse Spleen cells by flow cytometric analysis.
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