Anti-CD40 antibody

• WB

AbReview3189****

Western blot - Anti-CD40 antibody (AB13545)

This image is courtesy of an Abreview submitted on 10 August 2005.

All lanes:

Western blot - Anti-CD40 antibody (ab13545) at 1/1000 dilution

Lanes 1 - 2:

Control human HCAEC cells - whole cell lysate

Lanes 3 - 4:

Treated human HCAEC cells - whole cell lysate

Secondary

All lanes:

HRP conjugated Goat Anti-Rabbit IgG

Predicted band size: 30 kDa

Observed band size: 45 kDa

true

Exposure time: 20s

• WB

AbReview9985****

Western blot - Anti-CD40 antibody (AB13545)

The primary antibody was incubated with the membrane for 16 hours at 4°C.

All lanes:

Western blot - Anti-CD40 antibody (ab13545) at 1/5000 dilution

Lane 1:

Human B chronic lymphocytic leukaemia cells, nuclear fraction at 18 µg

Lane 2:

Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction at 18 µg

Lane 3:

Human B chronic lymphocytic leukaemia cells, membrane fraction at 18 µg

Lane 4:

Human B chronic lymphocytic leukaemia cells, cytosolic fraction at 18 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit

Predicted band size: 30 kDa

Observed band size: 42 kDa

true

Exposure time: 3min

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-CD40 antibody (AB13545)

All lanes:

Western blot - Anti-CD40 antibody (ab13545) at 1/200 dilution

Lane 1:

ESK4 cell lysate

Lane 2:

Molt 4 cell lysate

Predicted band size: 30 kDa

false

• WB

Unknown

Western blot - Anti-CD40 antibody (AB13545)

All lanes:

Western blot - Anti-CD40 antibody (ab13545) at 1/1000 dilution

All lanes:

Western blot - Recombinant human CD40 protein (Fc Chimera Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-cd40-protein-fc-chimera-active-ab83917'>ab83917</a>) at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 30 kDa

Observed band size: 72 kDa

true

Exposure time: 8min

• WB

CiteAb

Western blot - Anti-CD40 antibody (AB13545)

CD40 western blot using anti-CD40 antibody ab13545. Publication image and figure legend from Feng, L., Li, X. Q., et al., 2012, Virol J, PubMed 22925563.

ab13545 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab13545 please see the product overview.

Comparison of deferentially expressed CD40 using real-time PCR and Western blot. (a) mRNA changes of CD40 gene in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. (Note : Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test). (b) Detection of changes in protein expression by western blot with specific antibodies against CD40, β-tubulin, and CSFV glycoprotein E2 in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. The dimer of E1 and E2 (E1/E2) was detected with specific antibody against CSFV glycoprotein E2. (c) The quantification of changes in protein expression by the former western blot. The relative expression levels of CD40 were compared with that of β-tubulin. (Note : Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test).

false