Rabbit Polyclonal CD40 antibody. Suitable for WB, IHC-P and reacts with Pig, Human, Mouse, Rat, Guinea pig, Hamster, Monkey, Cow, Sheep, Rabbit samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human CD40 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- International journal of molecular sciences 24:2023CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed M Abu El-Asrar,Mohd I Nawaz,Ajmal Ahmad,Luna Dillemans,Mairaj Siddiquei,Eef Allegaert,Priscilla W Gikandi,Gert De Hertogh,Ghislain Opdenakker,Sofie StruyfPubMed 37958563
- Cells 12:2023Ectodomain Shedding by ADAM17 Increases the Release of Soluble CD40 from Human Endothelial Cells under Pro-Inflammatory Conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnton Klersy,Sören Meyer,Florian Leuschner,Thorsten Kessler,Markus Hecker,Andreas H WagnerPubMed 37566005
- Cancers 15:2023Generation of Orthotopic Patient-Derived Xenografts in Humanized Mice for Evaluation of Emerging Targeted Therapies and Immunotherapy Combinations for Melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi Yan,Caroline A Nebhan,Nabil Saleh,Rebecca Shattuck-Brandt,Sheau-Chiann Chen,Gregory D Ayers,Vivian Weiss,Ann Richmond,Anna E VilgelmPubMed 37509357
- Molecular medicine (Cambridge, Mass.) 28:1422022Icaritin inhibits neuroinflammation in a rat cerebral ischemia model by regulating microglial polarization through the GPER-ERK-NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZining Yu,Guangjun Su,Limei Zhang,Gaigai Liu,Yonggang Zhou,Shicai Fang,Qian Zhang,Tianyun Wang,Cheng Huang,Zhihua Huang,Liangdong LiPubMed 36447154
- Dentistry journal 10:2022Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Rogala,Zia Ali Khan,Linda Jackson-Boeters,Mark Roger DarlingPubMed 35323240
- NPJ precision oncology 6:62022Proximity of immune and tumor cells underlies response to BRAF/MEK-targeted therapies in metastatic melanoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi Yan,Sheau-Chiann Chen,Gregory D Ayers,Caroline A Nebhan,Joseph T Roland,Vivian L Weiss,Douglas B Johnson,Ann RichmondPubMed 35058553
- Journal of stroke 23:367-3762021Soluble CD40 Levels in Plasma Are Associated with Cardiovascular Disease and in Carotid Plaques with a Vulnerable Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnelie Shami,Andreas Edsfeldt,Eva Bengtsson,Jan Nilsson,Angela C Shore,Andrea Natali,Faisel Khan,Esther Lutgens,Isabel GonçalvesPubMed 34649381
- International journal of biological sciences 17:1088-11002021Genistein-3'-sodium sulfonate Attenuates Neuroinflammation in Stroke Rats by Down-Regulating Microglial M1 Polarization through α7nAChR-NF-κB Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoming Liu,Song Liu,Lijiao Xiong,Limei Zhang,Xiao Li,Xingling Cao,Jinhua Xue,Liangdong Li,Cheng Huang,Zhihua HuangPubMed 33867831
- AIDS (London, England) 34:2025-20352020Frequent expression of activation-induced cytidine deaminase in diffuse large B-cell lymphoma tissues from persons living with HIV.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVolodymyr Shponka,Candace Y Reveles,Sinthia Alam,Melba Jaramillo,Alanna Maguire,Lisa M Rimsza,Samantha KendrickPubMed 32773475
- Immunology letters 226:62-702020Injection of CD40 DNA vaccine ameliorates the autoimmune pathology of non-obese diabetic mice with Sjögren's syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Bo Zhou et. al.PubMed 32707129
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CD40 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P25942
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Expected |
| Rat | Predicted | Expected |
| Cow | Predicted | Expected |
| Dog | Predicted | Predicted |
| Guinea pig | Predicted | Expected |
| Hamster | Predicted | Expected |
| Monkey | Predicted | Expected |
| Pig | Tested | Expected |
| Rabbit | Predicted | Expected |
| Sheep | Predicted | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Monkey, Cow, Sheep, Rabbit, Guinea pig, Rat, Mouse, Hamster | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Guinea pig, Pig, Hamster, Monkey, Human, Cow, Sheep, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Alternative names
CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Recommended products
Rabbit Polyclonal CD40 antibody. Suitable for WB, IHC-P and reacts with Pig, Human, Mouse, Rat, Guinea pig, Hamster, Monkey, Cow, Sheep, Rabbit samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human CD40 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CD40 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P25942
- Purification technique
- Affinity purification
- Specificity
The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies.
- Concentration
- Purification notes
Peptide affinity purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Storage below -20°C is not recommended.
For maximum product recovery centrifuge the
product vial before removing cap. Shipped on gel packs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Western blot - Anti-CD40 antibody (ab13545)
This image is courtesy of an Abreview submitted on 10 August 2005.
All lanes: Western blot - Anti-CD40 antibody (ab13545) at 1/1000 dilution
Lanes 1 - 2: Control human HCAEC cells - whole cell lysate
Lanes 3 - 4: Treated human HCAEC cells - whole cell lysate
SecondaryAll lanes: HRP conjugated Goat Anti-Rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 45 kDa
Exposure time: 20s
Western blot - Anti-CD40 antibody (ab13545)
All lanes: Western blot - Anti-CD40 antibody (ab13545) at 1/200 dilution
Lane 1: ESK4 cell lysate
Lane 2: Molt 4 cell lysate
Predicted band size: 30 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-CD40 antibody (ab13545)
The primary antibody was incubated with the membrane for 16 hours at 4°C.
All lanes: Western blot - Anti-CD40 antibody (ab13545) at 1/5000 dilution
Lane 1: Human B chronic lymphocytic leukaemia cells, nuclear fraction at 18 µg
Lane 2: Human B chronic lymphocytic leukaemia cells, cytoskeletal fraction at 18 µg
Lane 3: Human B chronic lymphocytic leukaemia cells, membrane fraction at 18 µg
Lane 4: Human B chronic lymphocytic leukaemia cells, cytosolic fraction at 18 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 42 kDa
Exposure time: 3min
Western blot - Anti-CD40 antibody (ab13545)
All lanes: Western blot - Anti-CD40 antibody (ab13545) at 1/1000 dilution
All lanes: Western blot - Recombinant human CD40 protein (Fc Chimera Active) (Recombinant human CD40 protein (Fc Chimera Active) ab83917) at 1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 72 kDa
Exposure time: 8min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-CD40 antibody (ab13545)
CD40 western blot using anti-CD40 antibody ab13545. Publication image and figure legend from Feng, L., Li, X. Q., et al., 2012, Virol J, PubMed 22925563.
ab13545 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab13545 please see the product overview.
Comparison of deferentially expressed CD40 using real-time PCR and Western blot. (a) mRNA changes of CD40 gene in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. (Note: Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test). (b) Detection of changes in protein expression by western blot with specific antibodies against CD40, β-tubulin, and CSFV glycoprotein E2 in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. The dimer of E1 and E2 (E1/E2) was detected with specific antibody against CSFV glycoprotein E2. (c) The quantification of changes in protein expression by the former western blot. The relative expression levels of CD40 were compared with that of β-tubulin. (Note: Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test).
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