AB252557

Anti-CD40 antibody [BLR056F]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal CD40 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CD40 aa 200-300.

View Alternative Names

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

6 Images

Supplier Data

Immunocytochemistry - Anti-CD40 antibody [BLR056F] (AB252557)

FFPE section of Raji (Human Burkitt's lymphoma cell line) cells labeling CD40 using ab252557 at a 1/250 dilution in ICC.

Secondary : HRP-conjugated goat anti-rabbit IgG.

Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with ab252557 at a 1/250 dilution, followed by HRP-conjugated goat anti-rabbit IgG secondary. Substrate : Opal. Counterstain : DAPI (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

Supplier Data

Immunoprecipitation - Anti-CD40 antibody [BLR056F] (AB252557)

CD40 was immunoprecipitated from Raji (Human Burkitt's lymphoma cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252557 used for IP at 5 μl per reaction. For WB 1/1000 dilution.

Lane 1 : ab252557 IP in Raji whole cell lysate.
Lane 2 : Control IgG IP in Raji whole cell lysate.

Chemiluminescence detection : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD40 antibody [BLR056F] (ab252557)

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-CD40 antibody [BLR056F] (AB252557)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CD40 antibody [BLR056F] (ab252557) at 1/1000 dilution

Lane 1:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Lane 4:

GaMG (Human cell line) whole cell lysate at 50 µg

Lane 5:

Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 6:

A549 (Human lung carcinoma cell line) whole cell lysate at 50 µg

Lane 7:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg

Lane 8:

HDLM2 (Human cell line) whole cell lysate at 50 µg

Lane 9:

RKO (Human cell line) whole cell lysate at 50 µg

Lane 10:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 11:

MJ (Human cell line) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 30 kDa

false

Exposure time: 10s

Unconjugated

Anti-CD40 antibody [BLR056F] - BSA free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR056F

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human CD40 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P25942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "5 μl/1 mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

See full target information CD40

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1508047 PubMed40110131

2025

Amentoflavone protects against cisplatin-induced acute kidney injury by modulating Nrf2-mediated oxidative stress and ferroptosis and partially by activating Nrf2-dependent PANoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Jianqiang Hu,Yanmin Zhang,Xinxin Ci

Open medicine (Warsaw, Poland) 19:20241007 PubMed39308922

2024

Decreased BIRC5-206 promotes epithelial-mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Xu,Junjie Hu,Zhichao Ma,Wanyi Feng,Wei Gong,Shengmiao Fu,Xinping Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD40 antibody [BLR056F]

Immunocytochemistry - Anti-CD40 antibody [BLR056F] (AB252557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] (AB252557)

Immunoprecipitation - Anti-CD40 antibody [BLR056F] (AB252557)

Western blot - Anti-CD40 antibody [BLR056F] (AB252557)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise