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AB272087

Anti-CD40 antibody [BLR056F] - BSA free

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(1 Publication)

Rabbit Recombinant Monoclonal CD40 antibody. Suitable for IHC-P, IP, WB, ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CD40 aa 200 to C-terminus.

View Alternative Names

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

4 Images
Immunocytochemistry - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)

FFPE section of Raji (Human Burkitt's lymphoma cell line) cells labeling CD40 using ab252557 at a 1/250 dilution in ICC.

Secondary : HRP-conjugated goat anti-rabbit IgG.

Substrate : DAB.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).

Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)

CD40 was immunoprecipitated from Raji (Human Burkitt's lymphoma cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252557 used for IP at 5 µl per reaction. For WB 1/1000 dilution.

Lane 1 : ab252557 IP in Raji whole cell lysate.
Lane 2 : Control IgG IP in Raji whole cell lysate.

Chemiluminescence detection : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252557).

All lanes:

Immunoprecipitation - Anti-CD40 antibody [BLR056F] (<a href='/en-us/products/primary-antibodies/cd40-antibody-blr056f-ab252557'>ab252557</a>)

Predicted band size: 30 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR056F

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP, ICC

applications

Immunogen

Synthetic Peptide within Human CD40 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>" } } }
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Product details

ab272087 is the BSA-free version of ab252557.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 8.2 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
See full target information CD40
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1619194 PubMed41063980

2025

Mendelian randomization combined with single-cell sequencing analysis revealed prognostic genes related to myeloid cell differentiation in prostate cancer and experimental verification.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbai Chen,Jianxin Qiu,Wei Zhang,Zhiyong Nie,Xiaoping Gao,Gongquan Xu,Leiming Kang,Zhiming Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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