Rabbit Recombinant Monoclonal CD40 antibody. Suitable for IHC-P, IP, WB, ICC and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CD40 aa 200 to C-terminus.
Images
![Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (AB272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunoprecipitation-img57819.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130892.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)")
![Immunocytochemistry - Anti-CD40 antibody [BLR056F] - BSA free (AB272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunocytochemistry-img112757.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72848.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (AB272087)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human CD40 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25942
Reactivity data
Select an application| IHC-P | IP | WB | ICC | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Alternative names
CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Recommended products
Rabbit Recombinant Monoclonal CD40 antibody. Suitable for IHC-P, IP, WB, ICC and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CD40 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human CD40 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25942
- Clone number
- BLR056F
- Concentration
- Purification notes
Purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab272087 is the BSA-free version of Anti-CD40 antibody [BLR056F] ab252557.
This product is sold under License from Bethyl Laboratories, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (ab272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunoprecipitation-img57819.jpg "Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)")
Immunoprecipitation - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)
CD40 was immunoprecipitated from Raji (Human Burkitt's lymphoma cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Anti-CD40 antibody [BLR056F] ab252557 used for IP at 5 µl per reaction. For WB 1/1000 dilution.Lane 1: Anti-CD40 antibody [BLR056F] ab252557 IP in Raji whole cell lysate.
Lane 2: Control IgG IP in Raji whole cell lysate.Chemiluminescence detection: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-CD40 antibody [BLR056F] ab252557).
All lanes: Immunoprecipitation - Anti-CD40 antibody [BLR056F] (Anti-CD40 antibody [BLR056F] ab252557)
Predicted band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130892.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD40 with Anti-CD40 antibody [BLR056F] ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-CD40 antibody [BLR056F] ab252557).
![Immunocytochemistry - Anti-CD40 antibody [BLR056F] - BSA free (ab272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunocytochemistry-img112757.jpg "Immunocytochemistry - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)")
Immunocytochemistry - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)
FFPE section of Raji (Human Burkitt's lymphoma cell line) cells labeling CD40 using Anti-CD40 antibody [BLR056F] ab252557 at a 1/250 dilution in ICC.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-CD40 antibody [BLR056F] ab252557).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-blr056f-bsa-free-ab272087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72848.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [BLR056F] - BSA free (ab272087)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with Anti-CD40 antibody [BLR056F] ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (Anti-CD40 antibody [BLR056F] ab252557).
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- Visit the general protocols
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