Mouse Monoclonal CD40 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD40.
Images
![Flow Cytometry - Anti-CD40 antibody [C40/1605] (AB217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--flow-cytometry-img75084.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD40 antibody [C40/1605] (AB217900)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (AB217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8811.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (AB217900)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (AB217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13041.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (AB217900)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (AB217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61961.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (AB217900)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CD40. Database link P25942
Reactivity data
Select an application| Flow Cyt | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg for 106 Cells | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
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1 product for Alternative Version
Target data
Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Alternative names
Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CD40, TNFRSF5
Recommended products
Mouse Monoclonal CD40 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD40.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CD40. Database link P25942
- Clone number
C40/1605
- Purification technique
Affinity purification Protein A/G
- Light chain type
kappa
- Concentration
- Purification notes
ab217900 is purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
![Flow Cytometry - Anti-CD40 antibody [C40/1605] (ab217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--flow-cytometry-img75084.jpg "Flow Cytometry - Anti-CD40 antibody [C40/1605] (ab217900)")
Flow Cytometry - Anti-CD40 antibody [C40/1605] (ab217900)
Flow cytometry analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling CD40 with ab217900 at 2µg/10^6 cells followed by Goat Anti-Mouse IgG-CF488 (blue); isotype control (red).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8811.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen labelling CD40 with ab217900 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13041.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hodgkin's Lymphoma labelling CD40 with ab217900 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-c40-1605-ab217900--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61961.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [C40/1605] (ab217900)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD40 with ab217900 at 2 μg/ml.
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