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Rabbit Recombinant Monoclonal CD40 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-CD40 antibody [EPR18005-35] (AB188181), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-35] (AB188181), expandable thumbnail
  • Western blot - Anti-CD40 antibody [EPR18005-35] (AB188181), expandable thumbnail
  • Flow Cytometry - Anti-CD40 antibody [EPR18005-35] (AB188181), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Mouse
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).

Alternative names

Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, Cd40, Tnfrsf5

Recommended products

Rabbit Recombinant Monoclonal CD40 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18005-35

Purification technique

Affinity purification Protein A

Specificity

ab188181 recognises a different epitope to ab212058.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry - Anti-CD40 antibody [EPR18005-35] (ab188181), expandable thumbnail

    Flow Cytometry - Anti-CD40 antibody [EPR18005-35] (ab188181)

    Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD40 with ab188181 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-35] (ab188181), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-35] (ab188181)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma cell line) cells labeling CD40 with ab188181 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on A20 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

  • Western blot - Anti-CD40 antibody [EPR18005-35] (ab188181), expandable thumbnail

    Western blot - Anti-CD40 antibody [EPR18005-35] (ab188181)

    Blocking/Dilution: 5% NFDM/TBST.

    Exposure: 15 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 23404288 and PMID: 16709811).

    All lanes: Western blot - Anti-CD40 antibody [EPR18005-35] (ab188181) at 1/1000 dilution

    All lanes: A20 (mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 30 kDa

    Observed band size: 32-42 kDa

  • Flow Cytometry - Anti-CD40 antibody [EPR18005-35] (ab188181), expandable thumbnail

    Flow Cytometry - Anti-CD40 antibody [EPR18005-35] (ab188181)

    Flow cytometry staining of C57 BL/6 mouse splenocytes with ab188181 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab188181 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 5.0 μg/ml (1/388)) for 30min on ice. The cells were simultaneously stained with CD19.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

    Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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