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Rabbit Recombinant Monoclonal CD40 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse samples.

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Images

Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (AB212058), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (AB212058), expandable thumbnail
  • Western blot - Anti-CD40 antibody [EPR18005-4] (AB212058), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Mouse
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/600

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).

Alternative names

Tnfrsf5, Cd40, Tnfrsf5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor

Recommended products

Rabbit Recombinant Monoclonal CD40 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18005-4

Purification technique

Affinity purification Protein A

Specificity

ab212058 recognises a different epitope to ab188181.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (ab212058), expandable thumbnail

    Flow Cytometry - Anti-CD40 antibody [EPR18005-4] (ab212058)

    Flow cytometric analysis of A20 (mouse reticulum sarcoma cell line) cell line labeling CD40 with ab212058 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (ab212058), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [EPR18005-4] (ab212058)

    Immunofluorescent analysis of 100% methanol-fixed A20 (mouse reticulum sarcoma cell line) cells labeling CD40 with ab212058 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on A20 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-CD40 antibody [EPR18005-4] (ab212058), expandable thumbnail

    Western blot - Anti-CD40 antibody [EPR18005-4] (ab212058)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with the literature (PMID: 23404288 and PMID: 16709811).

    All lanes: Western blot - Anti-CD40 antibody [EPR18005-4] (ab212058) at 1/1000 dilution

    All lanes: A20 (mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 30 kDa

    Observed band size: 42 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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