Rabbit Recombinant Monoclonal CD40 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--western-blot-img182237.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85462.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995)")
![Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--western-blot-img169595.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img52471.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (AB271995)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Alternative names
CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40
Recommended products
Rabbit Recombinant Monoclonal CD40 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20540
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab271995 is the carrier-free version of Anti-CD40 antibody [EPR20540] ab213205.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--western-blot-img182237.jpg "Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)")
Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)
This data was developed using Anti-CD40 antibody [EPR20540] ab213205, the same antibody clone in a different buffer formulation:
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3 and 4: 30 seconds; Lane 5,6 and 7: 5 seconds.
The molecular weight observed is consistent with the literature (PMID: 23404288);
Negative control: Jurkat (PMID:10498643; PMID:15708600).
All lanes: Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995) at 1/2000 dilution
Lane 1: Human tonsil lysate at 20 µg
Lane 2: Human lymph node lysate at 20 µg
Lane 3: Human lymphoma lysate at 20 µg
Lane 4: Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Lane 5: Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Lane 6: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/4000 dilution
Lanes 4 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85462.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD40 with Anti-CD40 antibody [EPR20540] ab213205 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membranous and cytoplasmic staining on germinal center of human tonsil is observed [PMID: 10360965] [PMID: 7507299] [PMID:24452203].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD40 antibody [EPR20540] ab213205).
![Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--western-blot-img169595.jpg "Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)")
Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)
This data was developed using abab213205, the same antibody clone in a different buffer formulation.
Lanes 1 - 3: Merged signal (red and green). Green - Anti-CD40 antibody [EPR20540] ab213205 observed at 42 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
Anti-CD40 antibody [EPR20540] ab213205 was shown to react with CD40 in U-2 OS wild-type cells in Western blot. Loss of signal was observed when CD40 knockout sample was used. U-2 OS wild-type and CD40 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with Anti-CD40 antibody [EPR20540] ab213205 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995) at 1/2000 dilution
Lane 1: Wild-type U-2 OS whole cell lysate at 20 µg
Lane 2: CD40 knockout U-2 OS whole cell lysate at 20 µg
Lane 3: Raji whole cell lysate at 20 µg
Predicted band size: 30 kDa
Observed band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr20540-bsa-and-azide-free-ab271995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img52471.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR20540] - BSA and Azide free (ab271995)
Immunohistochemical analysis of paraffin-embedded human large B cell lymphoma tissue labeling CD40 with Anti-CD40 antibody [EPR20540] ab213205 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membranous staining on human large B cell lymphoma is observed [PMID: 7507299].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD40 antibody [EPR20540] ab213205).
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