Rabbit Recombinant Monoclonal CD40 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse samples. Cited in 2 publications.
Images
![Western blot - Anti-CD40 antibody [EPR23890-151] (AB252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--western-blot-img12356.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD40 antibody [EPR23890-151] (AB252428)")
![Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] (AB252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--immunoprecipitation-img11029.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] (AB252428)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (AB252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125075.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (AB252428)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (AB252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84858.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (AB252428)")
Publications
Filter by
- Biotechnology and bioengineering 120:767-7772022Membrane-coated nanoparticles for direct recognition by T cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeiran Li,Fanghua Li,Russell Urie,Elizabeth Bealer,Ramon Ocadiz Ruiz,Eiji Saito,Ali Turan,Esma Yolcu,Haval Shirwan,Lonnie D SheaPubMed 36515455
- Evidence-based complementary and alternative medicine : eCAM 2022:57528032022Antiliver Fibrosis Formula of Fuzheng Huayu Alleviates Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing-Qi Chang,Yi-Feng Pan,Jia-Yi Yang,Rong-Sheng Li,Chun-Lu Yuan,Ya-Fang Liao,Dan-Dan Zhang,Cheng-Hai LiuPubMed 36411840
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Although there is some immunogen cross reactivity, this Ab is not suitable for detection of human protein in WB. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Although there is some immunogen cross reactivity, this Ab is not suitable for detection of human protein in WB. |
Species Rat | Dilution info - | Notes Although there is some immunogen cross reactivity, this Ab is not suitable for detection of human protein in WB. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Alternative names
CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor
Recommended products
Rabbit Recombinant Monoclonal CD40 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23890-151
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD40 antibody [EPR23890-151] (ab252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--western-blot-img12356.jpg "Western blot - Anti-CD40 antibody [EPR23890-151] (ab252428)")
Western blot - Anti-CD40 antibody [EPR23890-151] (ab252428)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
LPS induces the expression of CD40 in Raw 264.7 cells (PMID: 23605514)
Exposure time: Lanes 1-2: 10 seconds Lane 3: 48 seconds.
All lanes: Western blot - Anti-CD40 antibody [EPR23890-151] (ab252428) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: RAW 264.7 treated with 1 μg/ml LPS (Lipopolysaccharide) for 6 hours whole cell lysate at 20 µg
Lane 3: Mouse lymph node tissue lysate at 20 µg
SecondaryLane 1: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Lanes 2 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 40 kDa
![Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] (ab252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--immunoprecipitation-img11029.jpg "Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] (ab252428)")
Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] (ab252428)
CD40 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 1 ug/ml LPS (Lipopolysaccharide) for 6 hours whole cell lysate with ab252428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252428 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 treated with 1 ug/ml LPS (Lipopolysaccharide) for 6 hours whole cell lysate 10 ug
Lane 2: ab252428 IP in RAW 264.7 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab252428 in RAW 264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
All lanes: Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] (ab252428)
Predicted band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (ab252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125075.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (ab252428)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (ab252428)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD40 with ab252428 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and cytoplasmic staining on mouse spleen (PMID: 25316820) is observed. The section was incubated with ab252428 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (ab252428), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-ab252428--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84858.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (ab252428)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] (ab252428)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CD40 with ab252428 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of mouse colon is observed. The section was incubated with ab252428 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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