Rabbit Recombinant Monoclonal CD40 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (AB273098), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-bsa-and-azide-free-ab273098--immunoprecipitation-img11029.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (AB273098)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (AB273098), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-bsa-and-azide-free-ab273098--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125075.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (AB273098)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (AB273098), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-bsa-and-azide-free-ab273098--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84858.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (AB273098)")
Publications
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- Cell death discovery 8:1172022MYC inhibition reprograms tumor immune microenvironment by recruiting T lymphocytes and activating the CD40/CD40L system in osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuo Jiang,Qianfeng Zhang,Yong Fan,Jia Li,Jitao Zhang,Wentao Wang,Jinzhu Fan,Yunshan Guo,Shichang Liu,Dingjun Hao,Yongxiang Wang,Lei Wang,Lequn ShanPubMed 35292660
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Expected | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Although there is some immunogen cross reactivity, this Ab is not suitable for detection of human protein in WB. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Although there is some immunogen cross reactivity, this Ab is not suitable for detection of human protein in WB. |
Species Rat | Dilution info - | Notes Although there is some immunogen cross reactivity, this Ab is not suitable for detection of human protein in WB. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).
Alternative names
CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor
Recommended products
Rabbit Recombinant Monoclonal CD40 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23890-151
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273098 is the carrier-free version of Anti-CD40 antibody [EPR23890-151] ab252428.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-bsa-and-azide-free-ab273098--immunoprecipitation-img11029.jpg "Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098)")
Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098)
CD40 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 1 ug/ml LPS (Lipopolysaccharide) for 6 hours whole cell lysate with Anti-CD40 antibody [EPR23890-151] ab252428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD40 antibody [EPR23890-151] ab252428 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: RAW 264.7 treated with 1 ug/ml LPS (Lipopolysaccharide) for 6 hours whole cell lysate 10 ug
Lane 2: Anti-CD40 antibody [EPR23890-151] ab252428 IP in RAW 264.7 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD40 antibody [EPR23890-151] ab252428 in RAW 264.7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD40 antibody [EPR23890-151] ab252428).
All lanes: Immunoprecipitation - Anti-CD40 antibody [EPR23890-151] (Anti-CD40 antibody [EPR23890-151] ab252428)
Predicted band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-bsa-and-azide-free-ab273098--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125075.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD40 with Anti-CD40 antibody [EPR23890-151] ab252428 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous and cytoplasmic staining on mouse spleen (PMID: 25316820) is observed. The section was incubated with Anti-CD40 antibody [EPR23890-151] ab252428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD40 antibody [EPR23890-151] ab252428).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098), expandable thumbnail](https://content.abcam.com/products/images/cd40-antibody-epr23890-151-bsa-and-azide-free-ab273098--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84858.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [EPR23890-151] - BSA and Azide free (ab273098)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CD40 with Anti-CD40 antibody [EPR23890-151] ab252428 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on stromal cells of mouse colon. The section was incubated with Anti-CD40 antibody [EPR23890-151] ab252428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD40 antibody [EPR23890-151] ab252428).
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