Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)

Rabbit Recombinant Multiclonal CD40 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt, IP and reacts with Human, Mouse samples.

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Images

Flow Cytometry - Anti-CD40 antibody [RM1193] - BSA and Azide free (AB317587), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt	IP
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Not recommended	Expected	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Target data

See full target information Cd40

Function

Receptor for TNFSF5/CD40LG (By similarity). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (PubMed:12881420).

Alternative names

CD40, Tnfrsf5, Cd40, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor

Recommended products

Rabbit Recombinant Multiclonal CD40 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt, IP and reacts with Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1193
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for mouse ICC and mouse IP.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab317587 is the carrier-free version of Anti-CD40 antibody [RM1193] ab317586.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

Flow Cytometry - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Jurkat(human T cell leukemia T lymphocyte from peripheral blood, Left) / Raji(human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cell.

Negative control: Jurkat.
Western blot - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Anti-CD40 antibody (Anti-CD40 antibody [RM1193] ab317586) staining at 1/1000 dilution is specific to human and mouse species. Anti-CD40 antibody (Anti-CD40 antibody ab13545) at 1/1000 dilution is bandy than Anti-CD40 antibody [RM1193] ab317586.
The molecular weight observed is consistent with the literature (PMID: 23404288).
Negative control: Jurkat (PMID:10498643; PMID:15708600).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 6: 103 seconds Lane 2-5: 92 seconds
All lanes: Western blot - Anti-CD40 antibody [RM1193] (Anti-CD40 antibody [RM1193] ab317586) at 1/1000 dilution
Lane 1: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 2: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
Lane 4: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 5: RAW 264.7 treated with /ml LPS (Lipopolysaccharide) for 6 hours whole cell lysate at 20 µg
Lane 6: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 42 kDa, 36 kDa
Immunoprecipitation - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
CD40 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with Anti-CD40 antibody [RM1193] ab317586 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD40 antibody [RM1193] ab317586 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Lane 2: Anti-CD40 antibody [RM1193] ab317586 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD40 antibody [RM1193] ab317586 in Raji whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-CD40 antibody [RM1193] (Anti-CD40 antibody [RM1193] ab317586) at 1/30 dilution
Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2: Anti-CD40 antibody [RM1193] ab317586 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 111s
Western blot - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Anti-CD40 antibody (Anti-CD40 antibody [RM1193] ab317586) staining at 1/1000 dilution is specific to human and mouse species. Anti-CD40 antibody (Anti-CD40 antibody ab13545) at 1/1000 dilution is weak than Anti-CD40 antibody [RM1193] ab317586 in mouse tissue lysate.
The molecular weight observed is consistent with the literature (PMID: 23404288).
The lysis conditions of the tissues could be further optimised to improve performance.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD40 antibody [RM1193] (Anti-CD40 antibody [RM1193] ab317586) at 1/1000 dilution
Lane 1: Human lymph node tissue lysate at 20 µg
Lane 2: Mouse thymus tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 42 kDa, 36 kDa
Exposure time: 136s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/500 (1.016 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse cardiac muscle. The section was incubated with Anti-CD40 antibody [RM1193] ab317586 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cardiac muscle. The section was incubated with Anti-CD40 antibody [RM1193] ab317586 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/500 (1.016 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse colon. The section was incubated with Anti-CD40 antibody [RM1193] ab317586 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/500 (1.016 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with Anti-CD40 antibody [RM1193] ab317586 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human hodgkin's lymphoma tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human hodgkin's lymphoma. The section was incubated with Anti-CD40 antibody [RM1193] ab317586 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human diffuse large B-cell lymphoma. The section was incubated with Anti-CD40 antibody [RM1193] ab317586 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of human colon in (A) Anti-CD40 antibody [RM1193] ab317586, non-specific staining on human colon in (B) Anti-CD40 antibody ab13545. The image on (A) Anti-CD40 antibody [RM1193] ab317586 is applied with Anti-CD40 antibody at 1/2000 dilution and (B) Anti-CD40 antibody ab13545 is applied with Anti-CD40 antibody at 1/4000 dilution.

The section was incubated with Anti-CD40 antibody [RM1193] ab317586 or Anti-CD40 antibody ab13545 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil in (A) Anti-CD40 antibody [RM1193] ab317586 and (B) Anti-CD40 antibody ab13545.

The image on (A) Anti-CD40 antibody [RM1193] ab317586 is applied with Anti-CD40 antibody at 1/2000 dilution and (B) Anti-CD40 antibody ab13545 is applied with Anti-CD40 antibody at 1/4000 dilution.

The section was incubated with Anti-CD40 antibody [RM1193] ab317586 or Anti-CD40 antibody ab13545 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-CD40 antibody [RM1193] - BSA and Azide free (ab317587)
This data was developed using Anti-CD40 antibody [RM1193] ab317586, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CD40 with Anti-CD40 antibody [RM1193] ab317586 at 1/50 (10.16 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing membranous staining in Raji cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

Negative control: Jurkat (PMID: 10498643, PMID: 15708600).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.